Tissue culture micropropagation of Douglas-fir |
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Authors: | Gina H Mohammed Kamlesh R Patel |
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Institution: | (1) AgriForest Technologies Limited, 2330 Enterprise Way, P.O. Box 178, VlY 7N5 Kelowna, British Columbia, Canada |
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Abstract: | A procedure was developed for plantlet production from embryos of Douglas-fir Pseudotsuga menziesii (Mirb.) Franco]. The Medium for Conifer Morphogenesis, used at full strength, and supplemented with 10 M benzyladenine for 17 days produced an average of 6.8 shoots on more than 90% of the embryos. The percentage of shoot-forming embryos as well as the average number of shoots per embryo varied significantly among eight seedlots. For secondary multiplication, 89% of the adventitious shoots produced axillary buds on MCM with 5 M benzyladenine. However, 0.5 M BA was more suitable for the elongation of axillaries. Rooting ranged from 0–87% depending upon the treatment. The highest percentage was obtained with a 7-week incubation in peat:perlite containing 1/5-strength medium, 1% sucrose and 2.7 M naphthaleneacetic acid, followed by 5 weeks on peat:perlite with 1/5-strength major and minor salts, full iron and organics, 1% sucrose and 0.1% charcoal. |
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Keywords: | Pseudotsuga menziesii tissue culture embryo explants N-benzyladenine root initiation auxins |
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