组织培养中用秋水仙素诱发黄花菜同源四倍体的研究

 在组织培养中用秋水仙素溶液诱发黄花菜同源四倍体的试验结果表明，该方法诱发频率高，能使加倍、选择和快速繁殖同步进行。在此基础上建立了同源四倍体黄花菜体细胞无性系变异的育种程序，并育成了国际上第一个食用黄花菜同源四倍体新品系。通过鉴定，该品系高产、优质、有重要推广价值。另外，对获得的黄花菜同源四倍体与对照二倍体试管苗进行了形态、细胞学及花粉粒等方面的观察比较。

A STUDY ON THE INDUCTION OF AUTOTETRAPLOID OF DAYLILY WITH COLCHICINE IN VITRO

The method used to induce the autotetraploid of daylily with colchicine in vitro,the comparative observations on characters of morphology and cytology of shoot and plantlet between the autotetrapoid and diploid are reported in this .paper.Their guard cells and pollen grains were also observed.Our research results indicated:this method had high indicated frequency,and it can take selection and rapid propagation to be done synchronally,and according to these resultes,a new breeding procedure of clonal lines of somatic cell of autotetraploid daylily was established.A new strain of autotetraploid of edible daylily was bred artificially for the first time in the world by this procedure,and the identifical results indicated that this new strain had good quality and high output,and it had very important significance to spread.This breeding procedure can shorten the breeding peroid and is suitable to induce these crop tetraploids which propagated themselves asexually with their rootstocks or bulbs.