螺旋藻提纯物含硒藻蓝蛋白的抗氧化和抗肿瘤活性(英文)

使用离子交换和凝胶过滤色谱法从富硒螺旋藻中提纯含硒藻蓝蛋白。通过含硒藻蓝蛋白与藻蓝蛋白对4种不同的自由基清除能力的比较,即总抗氧化能力、清除DPPH自由基、过氧化物阴离子和引发红细胞溶血的过氧化氢自由基能力测定,从而对含硒藻蓝蛋白的抗氧化活性进行评价。结果显示,经提纯后的含硒藻蓝蛋白在某一特定剂量下与藻蓝蛋白在清除ABTS、DPPH、过氧化物阴离子和AAPH自由基能力相比,都表现出更好的抗氧化作用。评价提纯后的含硒藻蓝蛋白和藻蓝蛋白在抑制人体恶性黑素瘤细胞(A-375)的生长和增殖的作用。结果表明,提纯后的含硒藻蓝蛋白和藻蓝蛋白的IC50分别在44.5μM和65.9μM能显著地抑制A-375细胞的增殖。使用流体血细胞计数分析和末端脱氧核苷酸转移酶和脱氧尿三磷酸缺口末端标记对A-375细胞凋亡的诱发进行测定。

Antioxidant and Anticancer Activities of Selenium-containing Phycocanin Purified from Spirulina Platensis

Selenium-containing phycocyanin (Se-PC) was purified from Se-enriched Spirulina platensis using ion exchange and gel filtration chromatography. Antioxidant activity of Se-PC was investigated and compared with phycocyanin (PC) by using four different free radical scavenging assays, namely, trolox equivalent antioxidant capacity (TEAC) assay, DPPH free radical scavenging assay, superoxide anion scavenging assay and assay for erythrocyte hemolysis mediated by peroxyl free radicals (AAPH assay) . The results indicated that the purified Se-PC exhibited better antioxidant activities than PC by scavenging the ABTS, DPPH, superoxide anion and AAPH free radicals in a dose-dependent manner. The anticancer activities of purified Se-PC and PC were tested on growth and multiplication of human malignant melanoma cell line (A-375) . The results indicate that Se-PC and PC significantly inhibit the proliferation of A-375 cells with IC50 values at 44.5 μ M and 65.9 μ M respectively. The induction of apoptosis in A-375 cells was evaluated by using flow cytometric analysis and the terminal deoxynucleotidyltransferase - mediated dUTP nick end labeling assay.