维吾尔药材刺山柑组织快繁无菌系建立的初步研究

目的]建立的刺山柑组织快繁无菌系.方法]根据不同部位、不同采集时间等,确定刺山柑外植体的采集时间、部位、灭菌剂和灭菌时间.结果]建立刺山柑的无菌体系时,选择晴天去采集外植体为好;带芽的茎段污染率高,腋芽的污染率低,选用腋芽作为外植体;操作台外的最佳消毒方法是饱和的洗洁精清洗后用自来水冲洗20 min;操作台内消毒对比10;次氯酸钠、2;双氧水、2;溴水、0.1;升汞,0.1;升汞灭菌效果好,而最佳操作台内最佳灭菌体系为75;酒精消毒10 s,以灭菌去离子水冲洗2次,后于0.1;升汞中浸泡9 min,以灭菌去离子水冲洗5次.结论]对外植体的采集时间晴天相对雨天污染率低,腋芽出芽率高,酒精和升汞双灭菌效果最佳.

Establishment of a Aseptic System of Medicinal Plant Capparis spinosa L. Explant in Vitro

【Objective】Establishment of a aseptic system of medicinal plant.【Method】 In this article,gathering time,type,biocide,disinfection time of explants in vitro with Capparis spinosa L.are studied.【Result】When establishing Capparis spinosa L.aseptic system,explants gathering should be shosen in the cloudless day;The pollution rate of the stems with a bud was high,but the pollution rate of a bud was low,the optimum explants are a bud segment in the cloudless day;The disinfection should first use the saturated laundry utility solution to soak outside the operation table,then flushes 20 minutes with the running water.When the inside of operation table was sterilized,it should be compared to 10% NaClO,2% H2O2,2% Br2,0.1% HgCl,0.1%HgCl solution has the best sterilization effect.The optimal system in the optimal operation sterilization table was 75% ethylal-cohol soak 10 seconds,the aseptic water flushes 2 times,then it was soaked with 0.1% mercuric chloride for 9 minutes,the aseptic water flushes 5 times.【Conclusion】Compared with the raining day,the explants of pollution rate is lower in the cloudless day.Growwing rate of bud is high.ethylal-cohol and HgCl had the best sterilization effect.