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| 卡特兰ACC氧化酶cDNA片断的克隆与序列分析 | |
| 引用本文： | 郑宝强,王雁,彭镇华,李晓华,齐靖,李莉.卡特兰ACC氧化酶cDNA片断的克隆与序列分析[J].河北农业大学学报,2008,31(6). |
| 作者姓名： | 郑宝强 王雁 彭镇华 李晓华 齐靖 李莉 |
| 作者单位： | 1. 中国林业科学研究院,林业研究所,国家林业局林木培育重点实验室,北京,100091 2. 国际竹藤网络中心,国家林业局竹藤科学与技术重点实验室,北京,100102 3. 河北农业大学,园艺学院,河北,保定,071001 |
| 摘 要： | 以卡特兰(Cattleya)花朵为试材,首先提取总RNA,并根据其它兰花的ACC氧化酶(1-aminocyclopropane-1-carboxylic acid oxidase,ACO)基因保守序列设计1对特异性引物,然后通过RT-PCR法,克隆得到一条卡特兰ACC氧化酶的cDNA片断,该片断长度为967 bp,共编码321个氨基酸残基。序列分析结果显示该克隆片断与已发表的其它兰花的ACC氧化酶基因序列同源性很高,均在85%以上,尤其与其近亲C.bicolor、C.inter-media、Laelia anceps的同源性均在95%以上。 |
| 关 键 词： | 卡特兰 ACC氧化酶 RT-PCR 序列分析 |
Cloning and sequencing analysis of cDNA encoding ACC oxidase from Cattleya |
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| ZHENG Bao-qiang,WANG Yan,PENG Zhen-hua,LI Xiao-hua,Qi Jing,LI Li.Cloning and sequencing analysis of cDNA encoding ACC oxidase from Cattleya[J].Journal of Agricultural University of Hebei,2008,31(6). | |
| Authors: | ZHENG Bao-qiang WANG Yan PENG Zhen-hua LI Xiao-hua Qi Jing LI Li |
| Institution: | ZHENG Bao-qiang1,WANG Yan1,PENG Zhen-hua1,LI Xiao-hua2,Qi Jing3,LI Li3 |
| Abstract: | Total RNA was extracted from flowers of Cattleya.According to the conserved acid sequence for ACC oxidase in other orchids,a pair of Oligo nucleotide primers were designed.Using RT-PCR method,a cDNA fragment about 967 base pair which encoded 321 predicted amino acid residues were amplified.The result of Blast showed the sequence presented a very high match with the ACO genes from the other orchids,and the homologue was higher than 85%,especially the relatives of C.bicolor,C.intermedia and Laelia anceps whose homologue was higher than 95%. |
| Keywords: | Cattleya ACC oxidase RT-PCR sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |