不同倍性白桦叶芽中IAA和ABA含量分析

[目的]初步比较二、三、四倍体白桦叶芽中IAA和ABA含量的差异,为研究植物内源激素在不同倍性白桦生长发育过程中所发挥的作用提供参考.[方法]利用HPLC法测定了二、三、四倍体白桦叶芽中IAA和ABA含量.[结果]对不同倍性白桦叶芽IAA与ABA含量的比较发现,多倍体白桦叶芽IAA含量均显著高于二倍体,而ABA含量则显著低于二倍体;单位质量的二、三、四倍体白桦叶芽中IAA/ABA的值分别为0.76、1.45、1.06.[结论]染色体倍数的增加对内源激素含量产生了重要影响.

Analysis of IAA and ABA in Leaf Buds of Different Ploidy Betula platyphylla Suk

[Objective] To compare differences of IAA and ABA in leaf buds of diploidy,triploidy and tetraploidy white birches,which will provide reference for studying the role of plant endogenous hormone in growth and development process of Betula platyphylla Suk..[Method] HPLC method was adopted to determine IAA and ABA content in leaf buds of Betula platyphylla Suk..[Result] The comparison results of IAA and ABA content in leaf buds of different ploidy Betula platyphylla Suk.showed that the IAA was significantly higher and the ABA was significandy lower in the polyploidy than those in the diploidy,respectively.Ratios of IAA/ABA in diploidy,triploidy and tetraploidy Betula platyphylla Suk.were 0.76,1.45,1.06.[Conclusion] The result demonstrated that chromosome doubling has a significant impact on the content of endogenous phytohormone.