Polycomb家族基因BmSu（z）12在家蚕幼虫中的表达研究

目的]研究家蚕Polycomb蛋白家族基因BmSu（z）12在家蚕幼虫中的表达情况.方法]根据家蚕基因组预测的BmSu（z）12基因序列,设计特异引物,利用qRT-PCR对该基因在家蚕不同龄期幼虫中的表达进行研究.结果]BmSu（z）12基因在5龄幼虫蜕皮后2d和变态前2d有较高表达,而在1～5龄取食中期表达量较低,表达特征具有一定的规律性.结论]BmSu（z）12基因在家蚕幼虫生长发育中具有重要作用,该研究为进一步研究家蚕SU（Z）12蛋白的功能奠定基础.

Study on Expression of Polycomb Group Gene BmSu(z)12 in Silkworm Larvae

Objective] The aim was to study the expression of polycomb group gene BmSu（z）12,in silkworm larvae.Method] Based on the predicted BmSu（z） 12 gene sequence in the genome of silkworm,the specific primers were designed.qRT-PCR was applied to analyze the expression of BmSu（z） 12 in different instar larvae of silkworm.Result] The expression level of BmSu（z） 12 was high in the first and the last 2 days of the 5th newly molted larvae,low expression was observed in the eating larvae of each instar.It showed regular expression patterns in the different developmental stages of larva.Conclusion] BmSu（z）12 may have important roles in the development of the silkworm larvae,the study will provide fundamental knowledge for further investigation of SU（Z） 12 protein function in silkworm.