| 马齿苋的组织培养和植株再生 |
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| 引用本文： | 王红艳,黄群策,秦广雍,霍裕平.马齿苋的组织培养和植株再生[J].河南农业科学,2006(5):84-87. |
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| 作者姓名： | 王红艳 黄群策 秦广雍 霍裕平 |
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| 作者单位： | 郑州大学物理工程学院离子束生物工程省重点实验室,河南,郑州,450052 |
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| 摘 要： | 用马齿苋的幼嫩叶片和茎段建立了马齿苋组织培养及植株再生系统。最适愈伤组织诱导培养基为MS 6-BA 0.5 mg/L 2,4-D 0.5 mg/L或2,4-D 1.0 mg/L,愈伤组织诱导率均可达100%。将生长良好的愈伤组织块转入分化培养基中以诱导不定芽的分化,其最适分化培养基为MS 6-BA 4.0 mg/L NAA 0.2 mg/L,分化率达到87.5%。再生苗在1/2MS IBA 2.0 mg/L的培养基上诱导生根,经过15d培养,100%生根成苗。
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| 关 键 词： | 马齿苋 组织培养 愈伤组织 植株再生 |
| 文章编号： | 1004-3268(2006)05-0084-04 |
| 收稿时间： | 2005-11-13 |
| 修稿时间： | 2005年11月13 |
Tissue Culture and Plantlet Regeneration from Portulaca oleracea L |
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| WANG Hong-yan,HUANG Qun-ce,QIN Guang-yong,HUO Yu-ping.Tissue Culture and Plantlet Regeneration from Portulaca oleracea L[J].Journal of Henan Agricultural Sciences,2006(5):84-87. |
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| Authors: | WANG Hong-yan HUANG Qun-ce QIN Guang-yong HUO Yu-ping |
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| Institution: | Provincial Key Laboratory of Ion Beam Bio-engineering,Physical College,Zhengzhou University,Zhengzhou 450052, China |
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| Abstract: | A system of tissue culture and plantlet regeneration with tender leaves and shoots from Portulaca oleracea L was set up.The favorable callus induing medium was MS 6-BA 0.5 mg/L 2,4-D 0.5 mg/L or 2,4-D 1.0 mg/L.The callus induction frequency could get to 100%.The favorable shoot inducing medium was MS 6-BA 4.0 mg/L NAA 0.2 mg/L and the differentiation frequency was 87.5%.The differentiated shoots were cultured on the root inducing medium of 1/2MS IBA 2.0 mg/L for 15 days and roots appeared.All the shoots could grow into plantlet. |
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| Keywords: | Portulaca oleracea L Tissue culture Callus Plantlet regeneration |
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