丁(鱼岁)养殖群体遗传多样性的ISSR分析 |
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引用本文: | 关建义,杨浩,陈欣,陈宏喜,杨太有. 丁(鱼岁)养殖群体遗传多样性的ISSR分析[J]. 安徽农业科学, 2009, 37(18): 8367-8369 |
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作者姓名: | 关建义 杨浩 陈欣 陈宏喜 杨太有 |
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作者单位: | 河南师范大学生命科学学院,河南新乡,453007;新乡医学院生命科学技术系,河南新乡,453003;河南师范大学生命科学学院,河南新乡,453007 |
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基金项目: | 河南省科技攻关项目,河南省教育厅自然科学研究项目,河南省动物学重点学科项目 |
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摘 要: | [目的]分析丁[鱼][岁]养殖群体的遗传多样性水平,为其种质资源的评价、保护和合理利用提供依据。[方法]利用简单重复序列区间扩增多态性(ISSR)分子标记技术。[结果]选用77个随机引物对其20个个体的基因组DNA进行PCR扩增,有18个引物可以扩增出稳定且清晰的条带,共扩增出115条稳定的DNA位点,片段大小在100-2000bp,其中多态性位点14个,多态位点比例为12.17%。个体间遗传距离为0-0.1376,平均为0.0329±0.0056。[结论]丁[鱼][岁]群体的遗传多样性水平较低。
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关 键 词: | 丁(鱼岁) 遗传多样性 ISSR |
ISSR Analysis of Genetic Diversity of Tinca tinca Cultured Population |
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Affiliation: | GUAN Jian-yi et al (College of Life Sciences, Henan Norrnai University, Xinxiang, Henan 453007) |
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Abstract: | [Objective] The study analyzed the genetic diversities level of Tinca tinca cultured population,which provided reference base for the evaluation,protection and utilization of its germplasm resources.[Method] The experiment was carried out by Inter-Simple Sequence Repeat(ISSR) of molecular marker technique.[Result] 20 genome DNA were selected to PCR amplify from 77 ones,18 ISSR primers can be amplified the stable stripes including 115 stable DNA sites and the fragments ranging from 100 to 2 000 bp,which had 14 polymorphism loci and the percentage of polymorphic loci was 12.17%.The genetic distance between individuals was from 0 to 0.137 6,and the average genetic distance was 0.032 9±0.005 6.[Conclusion] The results showed that the genetic diversity level of Tinca tinca was low. |
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Keywords: | ISSR |
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