| Development of Simple Functional Markers for Low Glutelin Content Gene 1 （Lgc1） in Rice （Oryza sativa） |
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| 作者单位： | Jiangsu High Quality Rice Research and Development Center, Institute of Food Crops, Jiangsu Academy of Agricultural Sciences,Nanjing 210014, China,＂ #These authors contributed equally to this paper |
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| 摘 要： |
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| 关 键 词： | 蛋白基因 水稻育种 分子标记 蛋白含量 Oryza PCR检测 开发 水稻品种 |
| 收稿时间： | 2010-02-03 |
Development of Simple Functional Markers for Low Glutelin Content Gene 1 (Lgc1) in Rice (Oryza sativa) |
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| CHEN Tao,TIAN Meng-xiang,ZHANG Ya-dong,ZHU Zhen,ZHAO Ling,ZHAO Qing-yong,LIN Jing,ZHOU Li-hui,WANG Cai-lin. Development of Simple Functional Markers for Low Glutelin Content Gene 1 (Lgc1) in Rice (Oryza sativa)[J]. Rice science, 2010, 17(3): 173-178. DOI: 10.1016/S1672-6308(09)60014-7 |
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| Authors: | CHEN Tao TIAN Meng-xiang ZHANG Ya-dong ZHU Zhen ZHAO Ling ZHAO Qing-yong LIN Jing ZHOU Li-hui WANG Cai-lin |
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| Affiliation: | Jiangsu High Quality Rice Research and Development Center, Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China |
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| Abstract: | Rice with low glutelin content is suitable as functional food for patients affected by kidney failure. Low glutelin-content gene Lgc1 in rice has a 3.5-kb deletion between two highly similar glutelin genes GluB4 and GluB5, which locates on the short arm of chromosome 2. To improve the selection efficiency in low glutelin-content rice breeding, two molecular markers designated as InDel-Lgc1-1 and InDel-Lgc1-2 were developed to detect the low glutelin-content gene Lgc1. A double PCR detection indicated that combined use of the two markers could easily distinguish the genotypes of Lgc1 from different rice varieties. Therefore, as a simple and low-cost technique, the molecular marker could be widely used to identify different varieties with Lgc1 gene and applied in marker-assisted selection of low glutelin-content rice. |
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