| 分蘖葱头总黄酮微乳中槲皮素的含量测定 |
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| 引用本文： | 武敏,王静,郭秋实,丁志英,孙波.分蘖葱头总黄酮微乳中槲皮素的含量测定[J].特产研究,2012,34(1):63-65. |
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| 作者姓名： | 武敏 王静 郭秋实 丁志英 孙波 |
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| 作者单位： | 1. 吉林大学药学院,长春,130021
2. 吉林大学第一医院,长春,130021 |
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| 基金项目： | 吉林省卫生厅项目(20080812) |
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| 摘 要： | 用高效液相色谱法测定分蘖葱头总黄酮微乳中的槲皮素含量.采用Shmisadezu LC-10A Tvp高效液相色谱仪,YMC-PackPro C18RS(I.D.250mm×4.6mm)色谱柱,流动相:甲醇-0.4％磷酸溶液(51:49)；检测波长:365nm；流速:1.0mL/min.结果表明,槲皮素在0.268～85.816μg/mL范围内线性关系良好,平均回收率98.03％,RSD 1.02％.该方法简单、快速、准确、重复性好,可用于测定分蘖葱头总黄酮微乳中的槲皮素含量.
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| 关 键 词： | 分蘖葱头总黄酮 高效液相色谱法 含量测定 |
Content Determination of Quercetin in Microemulsion of Fiavonoid-rich Allium cepa L.var.agrogatnm Don Extract |
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| WU Min
,
WANG Jing
,
GUO Qiu-shi
,
DING Zhi-ying
,
SUN Bo.Content Determination of Quercetin in Microemulsion of Fiavonoid-rich Allium cepa L.var.agrogatnm Don Extract[J].Special Wild Economic Animal and Plant Research,2012,34(1):63-65. |
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| Authors: | WU Min WANG Jing GUO Qiu-shi DING Zhi-ying SUN Bo |
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| Institution: | a(Jilin University a.School of Pharmacy;b.First Hospital,Changchun 130021,China) |
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| Abstract: | The aim of this study was to determine the contents of quercetin in microemulsion of flavonoid-rich allium cepa L.var.agrogatnm don extract.High performance liquid chromatograph(HPLC) was used in this experiment.The condition as follows: Shmisadezu LC-10A Tvp(250mm×4.6mm) was adopted,mobile phase consisted of methanol-water(51:49),the wavelength was 365nm,and the flow rate was 1.0mL/min.The calibration curve of quercetin was in good linearity with the range of 0.268～85.816μg(r=0.999 9).The average recovery was 98.03%,and RSD was 1.02%.The HPLC method is simple,stable,accurate and reproducible,and it can be used for determining the content of quercetin in microemulsion of flavonoid-rich allium cepa L.var.agrogatnm don extract. |
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| Keywords: | Flavonoid-rich allium cepa L var agrogatnm don extract HPLC Determination |
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