| 柱前衍生化高效液相色谱法分析银耳多糖的单糖组成 |
| |
| 引用本文： | 陈英红,姜翔之,罗浩铭,王颖,姜瑞芝.柱前衍生化高效液相色谱法分析银耳多糖的单糖组成[J].特产研究,2012,34(1):37-39,54. |
| |
| 作者姓名： | 陈英红 姜翔之 罗浩铭 王颖 姜瑞芝 |
| |
| 作者单位： | 1. 吉林省中医药科学院,长春,130012
2. 吉林大学中日联谊医院,长春,130051 |
| |
| 基金项目： | 国家十一五科技重大专项(2009ZX09103-333);吉林省科技发展计划项目(20100907);吉林省中医药管理局项目(2010-pt040) |
| |
| 摘 要： | 采用1-苯基-3-甲基-5-吡唑啉酮柱前衍生化反相高效液相色谱法,建立了5种常见单糖的最佳分离条件,并将该条件用于银耳多糖的单糖组成分析.银耳多糖中含有甘露糖、葡萄糖醛酸、葡萄糖、木糖、岩藻糖,摩尔比为3.894:1.264:0.044:1.000:0.265.该方法简单、快速、灵敏度高、重现性良好,可用于银耳多糖的质量控制.
|
| 关 键 词： | 银耳多糖 衍生化 高效液相色谱法 组成糖 |
Analysis of Monosaccharide Composition in Tremella Polysaccharides by Precolumn Derivatization HPLC |
| |
| CHEN Ying-hong
,
JIANG Xiang-zhi
,
LUO Hao-ming
,
WANG Ying
,
JIANG Rui-zhi.Analysis of Monosaccharide Composition in Tremella Polysaccharides by Precolumn Derivatization HPLC[J].Special Wild Economic Animal and Plant Research,2012,34(1):37-39,54. |
| |
| Authors: | CHEN Ying-hong JIANG Xiang-zhi LUO Hao-ming WANG Ying JIANG Rui-zhi |
| |
| Institution: | 1(1.Jilin Academy of Traditional Chinese Medicine and Material Medical Sciences,Changchun 130012,China;2.China-Japan Union Hospital,JiLin University,Changchun 130051,China) |
| |
| Abstract: | The reversed-phase high performance liquid chromatographic(HPLC) method of precolumn-derivatization with 1-phenyl-3-methyl-5-pyrazolone was used to establish the optimized separation condition of five traditional monosaccharides.And this optimized separation condition was used to analyze the monosaccharide composition of Tremella polysaccharides.The results of HPLC analysis showed that Tremella polysaccharides consisted of mannose,glucuronic acid,glucose,xylose,fucose with the molar ratio of 3.894:1.264:0.044:1.000:0.265.The HPLC method is simple,rapid,sensitive,and convenient.So it can be applied the quality control of Tremella polysaccharides. |
| |
| Keywords: | Tremella polysaccharides Derivatization High performance liquid chromatography Monosaccharide composition |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
