Isozyme and quantitative traits polymorphisms in European provenances of perennial ryegrass (Lolium perenne L) |
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Authors: | WM Ursla Fernando MD Hayward MJ Kearsey |
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Institution: | (1) School of Biological Sciences, University of Birmingham, Birmingham, B 15 2 TT, United Kingdom;(2) AFRC Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Dyfed, SY23 3EB, United Kingdom |
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Abstract: | Twenty populations of Lolium perenne originating from a range of habitats in Europe were compared for isoenzyme polymorphisms
and agronomically important quantitative traits in order to establish relationships of the levels of diversity with the origin
of each population and to assess their suitability to be included in the European core collection of Lolium germplasm. Forty
genotypes from each of the twenty populations and each genotype represented by three clonal propagules, were field planted
in a fully randomized spaced plant design and fifteen quantitative characters including yield, persistency, reproductive and
disease resistence characters were evaluated over a period of two years. Seven putative isozyme loci were assayed to compare
the allozyme divergence of populations. The results of the isozyme survey indicate that 71–100% of the loci were polymorphic,
2.3–3.0 alleles/locus and the gene diversity was varying from 0.234–0.410. Of the total allelic diversity 94% remained within
populations (Hs) whilst only 6% was distributed among populations (Dst). The differences between populations were determined on the basis of allele frequencies and multivariate analyses of quantitative
characters. Populations significantly differed in their allele frequencies at all loci analysed. Random mating was predominant
in all populations at most of the loci. The study revealed that the German accession BA 10998 was clearly distinct from the
rest both in quantitative characters and allele frequencies. German accession BA 11015 with the lowest gene diversity showed
the highest genetic variation for quantitative characters. However, no strict relationship was found between the genetic distance
and the geograpical distribution of the populations. Among the quantitative characters, flowering time showed a strong relationship
with the type of management practised at the collection site than their place of origin.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | quantitative traits isoenzyme variation Lolium perenne perennial ryegrass multivariate analysis |
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