| 实时荧光定量PCR检测血液标本中光滑念珠菌方法的建立 |
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| 引用本文： | 孔丹莉,周浩,王茜,艾云灿,何玉清,刘伟伟,杨杰,丁元林. 实时荧光定量PCR检测血液标本中光滑念珠菌方法的建立[J]. 湛江医学院学报, 2013, 0(5): 493-495 |
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| 作者姓名： | 孔丹莉 周浩 王茜 艾云灿 何玉清 刘伟伟 杨杰 丁元林 |
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| 作者单位： | 广东医学院公共卫生学院流行病与卫生统计教研室;南方医科大学附属南方医院;中山大学生命科学学院 |
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| 基金项目： | 广东省自然科学基金面上项目(No.S2012010008271);东莞市科技计划高等院校项目(No.2012108102058);东莞市医疗卫生重点项目(No.2012105102010);广东医学院2011年博士启动基金(No.XB1212) |
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| 摘 要： | 目的 探索快速可靠检测血液标本中光滑念珠菌的新方法.方法 采用真菌26S rDNA D1/D2区序列作为引物,单独和同时扩增光滑念珠菌及白色念珠菌与光滑念珠菌混合物,对血液标本进行荧光定量检测.结果 本方法不仅能够稳定的扩增出光滑念珠菌26S rDNA序列,并且能准确区分出混合物中的白色念珠菌与光滑念珠菌.结论 应用26SrDNA D1/D2区序列的荧光定量PCR是快速、敏感和特异的检测光滑念珠菌方法.
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| 关 键 词： | 光滑念珠菌 白色念珠菌 荧光定量PCR |
Real-time quantitative PCR for detection of blood Candida glabrata |
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| KONG Dan-li;ZHOU Hao;WANG Qian;AI Yun-can;HE Yu-qing;LIU Wei-wei;YANG Jie;DING Yuan-lin. Real-time quantitative PCR for detection of blood Candida glabrata[J]. Journal of Guangdong Medical College, 2013, 0(5): 493-495 |
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| Authors: | KONG Dan-li ZHOU Hao WANG Qian AI Yun-can HE Yu-qing LIU Wei-wei YANG Jie DING Yuan-lin |
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| Affiliation: | KONG Dan-li;ZHOU Hao;WANG Qian;AI Yun-can;HE Yu-qing;LIU Wei-wei;YANG Jie;DING Yuan-lin;Department of Epidemiology and Medical Statistics,Public Health School of Guangdong Medical College; |
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| Abstract: | Objective To investigate a rapid and reliable method for detection of blood Candida glabrata. Methods Real- time quantitative PCR was applied to detect C. glabrata and mixture of C. glabrata and C. albicans from blood using fungal primers D 1/D2 region of 26S rDNA. Results D 1/D2 region of 26S rDNA was stably amplified in C. glabrata, and the products of C. glabrata and C. albicans were dist/nguishable. Conclusion Real-time quantitative PCR using D 1/D2 region of 26S rDNA is a rapid, sensitive and specific method for detection of C. glabrata. |
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| Keywords: | Candida glabrata Candida albicans Real-time quantitative PCR |
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