| 反义寡核苷酸干涉家蚕非编码RNA的研究 |
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| 引用本文： | 刘宗才,黄蕾,阚云超,李丹丹. 反义寡核苷酸干涉家蚕非编码RNA的研究[J]. 安徽农业科学, 2013, 41(19): 8441-8442 |
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| 作者姓名： | 刘宗才 黄蕾 阚云超 李丹丹 |
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| 作者单位： | 中英·南阳洛桑昆虫生物学联合实验室,河南南阳473061 |
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| 摘 要： | [目的]研究反义寡核苷酸干涉家蚕非编码RNA表达的情况。[方法]构建Bm-15的反义寡核苷酸探针,通过脂质体转染昆虫Sf9细胞干涉Bm-15的表达。[结果]转染后细胞中Bm-15的表达量下降了63%,差异达显著水平,表明反义寡核苷酸探针对Bm-15基因表达有较好的抑制作用。[结论]该研究为进一步研究非编码RNA的功能奠定了良好的基础。
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| 关 键 词： | 家蚕 反义寡核苷酸 非编码RNA |
Study on Interference of Non-coding RNAs by Antisense Oligonucleotides in Silkworm |
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| Affiliation: | LIU Zong-cai et al (China-UK-NYNU2RRes Joint Libratory of Insect Biology, Nanyang Normal University, Nanyang, Henan 473061 ) |
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| Abstract: | [ Objective] The aim was to study the interference of non-coding RNAs by antisense oligonucleotides （ASO） in silkworm. [ Meth-od] The ASO probe of Bin-15 was designed, and transfected to St9 cells by liposome to interference the expression of Bin-15. [ Result] The expression of Bm-15 decreased by 63% after ASO transfection, and the difference reached significant level, which showed that the ASO probe had good inhibitory effect on Bin-15 gene expression. [ Conelusion] The study laid a good foundation for the further study on the function of non-codina RNAs. |
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| Keywords: | Silkworm Antisense oligonucleotides Non-coding RNAs |
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