| 黔产艾纳香黄酮抗氧自由基的研究 |
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| 引用本文： | 李琴山,刘洋. 黔产艾纳香黄酮抗氧自由基的研究[J]. 安徽农业科学, 2013, 41(19): 8449-8450 |
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| 作者姓名： | 李琴山 刘洋 |
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| 作者单位： | 1. 贵阳医学院检验系,贵州贵阳,550004
2. 北京工业大学生命科学与生物工程系,北京,100029 |
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| 基金项目： | 贵州省中医药管理局基金QZYY2010-2 |
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| 摘 要： | [目的]研究黔产艾纳香黄酮对氧自由基的清除能力。[方法]以黔产艾纳香为研究对象,提取其黄酮;采用邻二氨菲-Fe(Ⅱ)/H2O2体系产生羟自由基(·OH),用联苯三酚法在碱性溶液中自氧化反应产生超氧阴离子自由基(O2-·),并采用分光光度法测定黔产艾纳香黄酮对O2-·和·OH的清除作用。[结果]高剂量组的黔产艾纳香黄酮对O2-·具有明显的清除及抑制作用,对·OH也有一定的清除作用,清除率为(19.82%±0.17%)。[结论]黔产艾纳香黄酮对O2-·具有明显清除及抑制作用,对·OH也有一定的清除作用。
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| 关 键 词： | 艾纳香 分光光度法 超氧阴离子自由基 |
Study on Scavenging Action of Blumea flavanones on Oxygen Free Radical |
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| Affiliation: | LI Qin-shan et al ( Department of Laboratory of Guigang Medical University, Guiyang, Guizhou 550004) |
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| Abstract: | [ Objective] To study the scavenging action of Blumea flavanones on oxygen free radical. [ Method] Auto-oxidation in alkalcsent solution with the help of pyrogallol can produce O^2-·and the system of 1,10-phenathroline-Fe（ Ⅱ ）/H2O2 can generate · OH. Spectropho- tometry was used to observe the effects of Blumeaflavanones upon O^2-· and O^2-· OH in vitro. [ Result] High dosage Blumeaflavanones has ob-vious scavenging and inhibiting effect on O^2-· , and can reduce the level of · OH by （19.82%-0. 17% ）. [ Conclusion] Guizhou＇ s Blumea flavanones could scavenge and inhibit O^2-· significantly and could scavenge · OH as well. Key words Blumea flavanones ; Spectrophotometry ; Superoxide anion radical |
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| Keywords: | Blumea flavanones Spectrophotometry Superoxide anion radical |
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