枇杷花疫病病原菌鉴定(英文)

    枇杷花疫病于2007年在浙江省德清县严重发生,造成枇杷花穗枯萎和死亡.为了鉴定其病原菌,从受害枇杷花穗上分离到6个病原菌菌株.依据病原菌的形态学和培养特征,该病原菌被鉴定为灰葡萄孢菌(Botrytis cinerea),其有性态为富氏葡萄孢盘菌(Botryotinia fuckeliana Whetzel.).通过核糖体DNA ITS序列分析,分离菌株序列与富氏葡萄孢盘菌序列100%同源,进一步证明侵染枇杷花穗的病原菌是灰葡萄孢菌(Botrytis cinerea).致病性试验结果表明,B.cinerea 能侵染枇杷花穗,可引起与自然寄主上相似的病害症状.

Identification of causal agent of blossom blight of loquat in Zhejiang Province,China

The blossom blight of loquat occurred severely in 2007 in loquat orchards in Deqing County,Jiaxing District,Zhejiang Province of China,causing the flower spike wilt and death of loquat.To identify the causal agent,six Botrytis sp.isolates were obtained from the diseased flower spikes of loquat.Based on morphological and cultural characters,the isolates were identified as Botrytis cinerea Pers,and its teleomorph is Botryotinia fuckeliana Whetzel.By analysis of nucleotide sequences of the ITS regions of ribosomal DNA (ITS1 and ITS2) and the 5.8S RNA gene,the sequences of isolates were 100% identical to that of B.fuckeliana,further confirming that the pathogen was B.cinerea.Pathogenicity test showed that B.cinerea isolates were able to infect the flower spikes of loquat,causing the symptoms to be similar to natural hosts.