Mutagenic induction of double-podding trait in different genotypes of chickpea and their characterization by STMS marker |
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Authors: | H. Ali T. M. Shah N. Iqbal B. M. Atta M. A. Haq |
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Affiliation: | Mutation Breeding Division, Nuclear Institute for Agriculture &Biology (NIAB), P.O. Box 128, Jhang Road, Faisalabad, Pakistan;Corresponding author, E.mail: |
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Abstract: | A gene that confers double-podding in chickpea is considered to be important for breeding higher yielding cultivars. Double-podded mutants were produced from five desi- and four kabuli-type chickpea genotypes through induced mutations and stabilty was checked up to M13 generation. Desi-type produced higher number of mutants as compared with kabuli-type. The inheritance studies in induced mutants of six genotypes showed that the double-podded trait was governed by single recessive gene. Different genotypes and their double-podded mutants were also characterized through sequence-tagged microsatellite site marker, TA-80. Allelic variations were found in single-podded genotypes and eight different alleles were identified, while for double-poddedness no allelic variants were found in all the analysed mutants. Addition of bases in the double-podded mutants showed that there might be involvement of transposable elements in the production of double-podded mutants through mutagens. |
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Keywords: | double-podding molecular marker induced mutants chickpea |
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