中国北方地区甘草根瘤菌表型及遗传多样性研究

【目的】了解中国北方地区甘草根瘤菌的生物多样性。【方法】采用表型数值分类、16S rDNA PCR-RFLP分析和BOX-PCR指纹图谱分析的方法对北方地区的甘草根瘤菌进行表型、遗传多样性分析。【结果】供试菌株在数值分类聚类分析中约85％的相似水平上产生2个表观群，有11株菌未与已知参比菌株聚群。16S rDNA PCR-RFLP分析表明，供试的20株菌共产生14种遗传型，表现出丰富的遗传多样性。BOX-PCR指纹图谱分析进一步证明与甘草共生的根瘤菌的基因组也具有多样性。【结论】在中国北方地区与甘草共生的根瘤菌在Sinorhizobium、Rhizobium和Mesorhizobium属中均有分布。

Phenotypic and Genetic Diversity of Rhizobia Associated with Glycyrrhiza spp. Grown in Northern Regions of China

[Objective] The biodiversity of rhizobia from Glycyrrhiza spp. in northern regions of China was studied. [Method] Twenty isolates from Glycyrrhiza spp. were characterized by numerical taxonomy, 16S rDNA PCR-RFLP analysis and BOX-PCR fingerprinting to describe the phenotypic and genetic diversity. [Result] There were two clusters at 85% similarity by numerical taxonomy and 11 strains are separate from reference strains. Fourteen genotypes of tested strains were obtained from the tested 20 strains and analyzed by 16S rDNA PCR-RFLP. The diversity of genomic level was described by BOX-PCR fingerprinting. [Conclusion] All analyzed results of analysis desmonstrated that the symbiotic bacteria associated with Glycyrrhiza spp. could be found in the genera of Sinorhizobium, Rhizobium and Mesorhizobium.