| 骆驼伊氏锥虫病LAMP实验诊断初报 |
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| 引用本文： | 杨帆,王振宝,王常汉,巴音查汗.骆驼伊氏锥虫病LAMP实验诊断初报[J].中国兽医寄生虫病,2008,16(3):23-25. |
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| 作者姓名： | 杨帆 王振宝 王常汉 巴音查汗 |
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| 作者单位： | 新疆农业大学动物医学学院,乌鲁木齐,830052 |
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| 摘 要： | 目的为了探索伊氏锥虫病诊断的新方法。方法采用环状介导等温DNA扩增法（LAMP），分别对随即采样的14峰骆驼进行伊氏锥虫病诊断，并与血液涂片法对比。结果LAMP与血液涂片法检测得出的阳性率分别为35．71％和14．29％。结论LAMP可用来检测骆驼伊氏锥虫病，并且LAMP在等温条件下进行，不需要复杂的仪器，为临床检测锥虫病提供了一个快速简便的分子生物学方法。
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| 关 键 词： | 骆驼 伊氏锥虫病 LAMP 诊断 |
| 文章编号： | 1005-0868(2008)03-0023-03 |
| 修稿时间： | 2008年2月26日 |
LAMP FOR DETECTION OF TRYPANOSOMA EVANSI IN THE CAMELS |
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| YANG Fan,WANG Zhen-bao,WANG chang-han,Bayinchahan.LAMP FOR DETECTION OF TRYPANOSOMA EVANSI IN THE CAMELS[J].Chinese Journal of Veterinary Parasitology,2008,16(3):23-25. |
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| Authors: | YANG Fan WANG Zhen-bao WANG chang-han Bayinchahan |
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| Institution: | YANG Fan, WANG Zhen-bao, WANG chang-han, Bayinchahan ( College of Animal Medicine,Xinjiang Agricultural University, Urumqi 830052, China) |
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| Abstract: | Objective In order to explore an effective method of diagnosis for trypanosomatosis. Methods Fourteen camels are diagnosed by the method of the Loop-mediated isothermal amplification（LAMP） and traditional blood smear , then the two detection results were compared . Results The positive rates of Trypanosoma evansi were 35.71% and 14.29% detected by LAMP and blood smears respectively. Conclusion LAMP could be applied to diagnose the trypanosomatosis of camels, which performed under isothermal condition without special apparatus. The study provide a molecular biological method to detect trypanosomatosis in clinic rapidly and simply. |
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| Keywords: | Camels trypanosomatosis LAMP Diagnosis |
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