禽流感基因诊断技术研究进展

禽流感是由流感病毒引起的一种人和动物的高度传染性疾病，不但造成养禽业的巨大经济损失，还对人类公共卫生安全存在威胁。因此，快速、准确的基因诊断技术对于禽流感的防控显得非常重要。近年来，主要有RT—PCR技术、荧光定量RT—PCR技术、核酸依赖扩增技术（NASBA）以及基因芯片等基因诊断技术。文章就这些方法的基本原理、检测优点以及应用现状进行综述，以期为有效控制禽流感的发生与流行提供有力技术支撑。

Progress on Avian Influenza Gene Diagnosis Technology

Avian influenza is caused by the avian influenza virus and is a highly contagious disease of humans and animals,not only caused poultry industry huge economic losses,but also caused human security presence in public health threat.Therefore,rapid and accurate diagnosis of gene technology for the prevention and control of avian influenza is very important.At present,there are genetic diagnosis such as RT-PCR technique,fluorescence quantitative RT-PCR technology,reliance on the nucleic acid amplification technology(NASBA) and the new microarray technology.The paper reviewed the basic principles of these methods,testing and application of their merits,with a view to effectively control the avian influenza pandemic outbreak and to provide effective technical support.