Proteomic analysis of the G-layer in poplar tension wood |
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Authors: | Tomomi Kaku Satoshi Serada Kei’ichi Baba Fumio Tanaka Takahisa Hayashi |
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Institution: | (1) Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan;(2) National Institute of Biomedical Innovation, Ibaraki, Osaka 567-0085, Japan |
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Abstract: | Angiosperm trees bend their stems by forming tension wood at the upper side of leaning stems. Most tension wood has a cellulose-rich
G-layer in the innermost surface of the fiber cell wall. Strong tensile stress is considered to occur in the G-layer. This
study undertook to identify the proteins involved in G-layer formation and function through a proteomic analysis of G-layer-localized
protein. G-layers of poplar were loosened by sonication and isolated as doughnut-shaped pieces of thinly sliced transverse
sections. The proteins, once extracted with urea/detergent solution, were separated by two-dimensional polyacrylamide gel
electrophoresis, and 110 spots were subjected to liquid chromatography tandem mass spectrometry (LC/MS/MS). A database search
for these spots’ mass spectrum patterns identified 72 proteins. In addition, all peptide digestion mixtures of G-layer proteins
were separated by strong cation exchange chromatography and 39 proteins were identified using LC/MS/MS analysis. Proteins
involved in wall formation, such as lignin biosynthesis-related protein, xyloglucan endotransglucosylase, and fasciclin-like
arabinogalactan protein, were notably detected in the G-layer. |
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Keywords: | G-layer Populus Proteomics Tension wood |
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