重庆南瓜病毒病病原ELISA 检测及CMV 变异分析

 利用7 种植物病毒的抗体，对采自重庆7 个区县的67 份南瓜病毒病样品进行了抗原直接包被酶联免疫吸附分析（ACP-ELISA）检测。检测结果表明，在这67 份样品中，至少59 份感染了所要检测病毒，其中，感染黄瓜花叶病毒 (Cucumber mosaic virus, CMV)、烟草花叶病毒 (Tobacco mosaic virus,TMV)、番茄花叶病毒 (Tomato mosaic virus, ToMV)、芜菁花叶病毒 (Turnip mosaic virus, TuMV)、蚕豆萎蔫病毒2 号 (Broad bean wilt virus 2, BBWV-2)、马铃薯Y 病毒 (Potato virus Y, PVY)和马铃薯X 病毒(Potato virus X, PVX) 的样品分别为80.60%、77.61%、74.63%、82.09%、76.12%、83.58%和55.22%。在检测呈阳性的59 份样品中有93.22%的样品受到2 种以上病毒的复合侵染，而受7 种病毒复合侵染的样品高达60.00%，表明在南瓜上病毒复合侵染现象相当严重。进一步对54 份受CMV 侵染的南瓜样品检测发现，CMV 亚组Ⅰ (CMVⅠ) 病毒侵染的样品有49 份，占90.74%，CMV 亚组Ⅱ (CMVⅡ) 病毒侵染的样品为11 份，占20.37%；其中6 份（11.11%）样品检测到CMVⅠ和CMVⅡ的复合侵染。以免疫捕获反转录PCR (IC-RT-PCR) 扩增了一个CMVⅠ分离物 (CMV-CQ) 近全长CP 基因并进行序列分析，结果证实其属于CMV 亚组Ⅰ型，其核苷酸序列与国内CMVⅠ型赤豆分离物 (CMV-RB) 的同源性最高，为98.1%。

ELISA Detection of Infectious Pathogenic Viruses and Variation of CMV from Squash in Chongqing

The 67 squash samples with viral symptoms collected from seven squash-growing counties in Chongqing were detected by antigen-coated plate(ACP)-ELISA with antibodies against seven plant viruses.The results showed that at least 59 samples with in these 67 squash samples were infected by the viruses,of which 80.60%,77.61%、70.15%,82.09%、76.12%、83.58% and 55.22% of the samples were infected by Cucumber mosaic virus(CMV),Tobacco mosaic virus(TMV),Tomato mosaic virus(ToMV),Turnip mosaic virus(TuMV),Broad bean wilt virus 2(BBWV-2),Potato virus Y(PVY)and Potato virus X(PVX),respectively.Among 59 positive samples,93.22%of the samples were mix-infected with more than two viruses,and about 60% samples were mix-infected by the above seven viruses,indicating the mix-infections of viruses on squash were severely.Moreover,the results showed that among 54 samples infected by CMV,49(90.74%)samples were infected by CMV subgroup Ⅰ(CMV Ⅰ)and 12(22.22%)samples by CMV subgroup.Comparison of the nucleotide sequences showed that it has the highest identity(98.1%)with the previously reported CMV Ⅰ isolate infecting Phaseolus angularis(CMV-RB)from China.