| 鲤鱼肌肉中胶原蛋白酶的分离纯化与性质分析 |
| |
| 引用本文： | 王诚,张超群,吴海龙,黄身镰,曹敏杰.鲤鱼肌肉中胶原蛋白酶的分离纯化与性质分析[J].厦门水产学院学报,2013(4):261-266. |
| |
| 作者姓名： | 王诚 张超群 吴海龙 黄身镰 曹敏杰 |
| |
| 作者单位： | [1]集美大学生物工程学院,福建厦门361021 [2]福建省高校水产科学技术与食品安全重点实验室,福建厦门361021 |
| |
| 基金项目： | 国家自然科学基金资助项目（31071519） |
| |
| 摘 要： | 利用明胶酶谱法,在鲤鱼肌肉中检测到了一种具有分解胶原蛋白能力的丝氨酸蛋白酶.通过硫酸铵盐析、DEAE-Sephacel和Phenyl-Sepharose等一系列柱层析法,纯化得到了分子质量为70 ku,能够降解胶原蛋白的丝氨酸蛋白酶（Collagenolytic Serine Proteinase,CSP）.性质分析表明,CSP在弱碱性条件下显示活性,最适温度为30℃;丝氨酸蛋白酶抑制剂特异抑制CSP的活性.CSP能有效地分解鲤鱼肌肉中的I型胶原蛋白,提示其可能在鱼体死后肌肉胶原蛋白降解中发挥着重要的作用.
|
| 关 键 词： | 丝氨酸蛋白酶 纯化 降解 I型胶原蛋白 |
Identification and Characterization of a Collagenolytic Proteinase from the Skeletal Muscle of Common Carp （Cyprinus carpio） |
| |
| Authors: | WANG Cheng ZHANG Chao-qun WU Hai-long HUANG Shen-lian CAO Min-jie |
| |
| Institution: | 1. College of Biological Engineering, Jimei University, Xiamen 361021, China; 2. Key Laboratory of Science and Technology for Aquaculture and Food Safety (Jimei University), Fujian Province, Xiamen 361021, China) |
| |
| Abstract: | A collagenolytic serine proteinases （CSP） from common carp muscle was identified and puri-fied to high purity by ammonium sulfate fractionation and chromatographies on DEAE - Sephacel and Phenyl-Sepharose. The molecular mass of the CSP was 70 ku as estimated by SDS - PAGE. CSP revealed high activ-ity at a slightly alkaline pH range and optimum temperature of 30 ℃. Serine proteinase inhibitors such as pe-fabloc SC, leupeptin and benzamindine suppressed the activity of CSP specifically. CSP hydrolyzed type I collagen effectively, indicating CSP was involved in the proteolytic breakdown of collagens in fish muscle dur-ing postmortem tenderization. |
| |
| Keywords: | serine proteinase purification degradation Ⅰ collagen |
| 本文献已被 维普 等数据库收录！ |
|
