| 一个新的家蚕BmLITAF基因的电子克隆及序列分析 |
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| 引用本文： | 刘玉生,陈健,聂作明,吕正兵,王丹,吴祥甫,张耀洲.一个新的家蚕BmLITAF基因的电子克隆及序列分析[J].蚕业科学,2007,33(4):562-567. |
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| 作者姓名： | 刘玉生 陈健 聂作明 吕正兵 王丹 吴祥甫 张耀洲 |
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| 作者单位： | 浙江理工大学生命科学学院生物化学研究所,杭州,310018 |
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| 基金项目： | 国家高技术研究计划863项目
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国家重点基础研究发展计划(973计划)
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浙江省自然科学基金 |
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| 摘 要： | 在对家蚕蛹cDNA文库的测序中发现了脂多糖诱导肿瘤坏死因子α的转录激活因子(lipopolysaccharide-induced tumor necrosis factor-α factor,LITAF)的EST序列(GenBank登录号AADK01016184)。经过比对发现BmLI-TAF全长为981 bp,由97 bp的5′端非翻译区序列(5′UTR)、390 bp的开放读码框(ORF)和494 bp的3′端非翻译区序列(3′UTR)组成。用电子克隆方法对BmLITAF进行基因结构分析发现,此基因由3个外显子和2个内含子组成,编码129个氨基酸。对BmLITAF蛋白进行疏水性、跨膜结构和信号肽分析的结果显示,BmLITAF具有跨膜结构域。根据BmLITAF的电子克隆序列由家蚕基因组PCR扩增获得BmLITAF基因,将其克隆到pGEM-T-easy载体中,测序验证与电子克隆结果一致。
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| 关 键 词： | 家蚕 脂多糖诱导的肿瘤坏死因子α的转录激活因子 电子克隆 序列分析 |
| 文章编号： | 0257-4799（2007）04-0562-06 |
| 收稿时间： | 2007-04-03 |
| 修稿时间： | 2007年4月3日 |
In Silico Cloning and Sequence Analysis of a Novel Gene,BmLITAF,From Silkworm, Bombyx mori |
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| LIU Yu-Sheng,CHEN Jian,NIE Zuo-Ming,L Zheng-Bing,WANG Dan,WU Xiang-Fu,ZHANG Yao-Zhou.In Silico Cloning and Sequence Analysis of a Novel Gene,BmLITAF,From Silkworm, Bombyx mori[J].Acta Sericologica Sinica,2007,33(4):562-567. |
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| Authors: | LIU Yu-Sheng CHEN Jian NIE Zuo-Ming L Zheng-Bing WANG Dan WU Xiang-Fu ZHANG Yao-Zhou |
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| Institution: | LIU Yu-Sheng,CHEN Jian,NIE Zuo-Ming,L(U) Zheng-Bing,WANG Dan,WU Xiang-Fu,ZHANG Yao-Zhou |
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| Abstract: | The EST of lipopolysaccharide-induced tumor necrosis factor-α factor(LITAF,GenBank accession no.AADK01016184) was found through searching cDNA bank of silkworm pupae.The full-length of the gene is 981 bp,which contains 5′ UTR(97 bp),ORF(390 bp) and 3′ UTR(494 bp).Genome structure of BmLITAF is composed of three exons and two introns and encodes 129 amino acids.Moreover,there is an transmembrane structure domain in BmLITAF.According to the in silico clone sequence of BmLITAF,the gene BmLITAF got from genome of Bombyx mori by PCR and cloned to pGEM-T-easy vector.The sequencing result showed that the clone sequence was identical with in silico clone sequence. |
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| Keywords: | Bombyx mori BmLITAF In silico cloning Sequence analysis |
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