不同温湿度处理对烤烟陈化过程中酶活性的影响

利用智能恒温恒湿培养箱通过控制温湿度条件,研究不同温湿度处理对烟叶陈化过程中多酚氧化酶、过氧化物酶、脂氧合酶和苯丙氨酸裂解酶活性的影响.结果表明:(1)随着陈化时间的延长,烟叶中各酶活性均呈现先上升后下降的趋势.(2)在不同温湿度条件下,陈化21个月时各酶活较陈化初始酶活降低幅度有所不同,其中多酚氧化酶活降幅最大,在中温高湿处理酶活下降82.93%.(3)在高温中湿、中温高湿和中温中湿条件下各酶活性在陈化前期上升幅度较大,在中后期下降幅度较大.(4)在整个陈化进程中,不同温湿度处理相比较,多酚氧化酶活降低幅度表现为中温高湿＞中温中湿＞高温中湿＞低温中湿＞中温低湿＞对照;过氧化物酶活降幅为中温高湿＞高温中湿＞中温中湿＞中温低湿＞对照＞低温中湿;脂氧合酶活降幅表现为中温中湿＞高温中湿＞中温高湿＞中温低湿＞对照＞低温中湿;苯丙氨酸裂解酶降低幅度为高温中湿＞中温高湿＞中温中湿＞中温低湿＞低温中湿＞对照.

Influence of Different Temperature-Humidity Treatments on Enzymatic Activity during Flue-cured Tobacco Aging

The changing rules of PPO,POD,LOX and PAL activities duringflue-cured tobacco aging were studied by using aptitude-constant-temperature-and-constant-moisture-trunk to control the temperature and humidity conditions.The results indicated that:(1) each enzymatic activity went up firstly and then went down during the whole aging stage;(2) enzymatic activities descending ranges of different treatments were different when aged 21 months,and PPO activity dropped the most,which decreased by about 82.93% in C treatment;(3)enzymatic activities of treatments E,C,B had higher increasing ranges in the early aging days,and had higher descending ranges in midanaphase;(4)in the whole aging process,by comparing the treatments with each other,the degrading ranges of PPO activity showed the order that C>B>E>D>A>CK;the degrading ranges of POD activity showed the order that C>E>B>A>CK>D;the degrading ranges of LOX activity showed the order that B>E>C>A>CK>D;the degrading ranges of LOX activity showed the order that E>C>B>A>D>CK.