| 牛尿苷酸合酶缺乏症PCR-RFLP检测方法的建立 |
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| 引用本文： | 王洪梅,李建斌,高运东,韩波,王长法,刘文浩,仲跻峰.牛尿苷酸合酶缺乏症PCR-RFLP检测方法的建立[J].中国奶牛,2006(3):6-8. |
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| 作者姓名： | 王洪梅 李建斌 高运东 韩波 王长法 刘文浩 仲跻峰 |
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| 作者单位： | 1. 山东省农业科学院奶牛研究中心,济南,250100
2. 山东奥克斯生物技术有限公司,济南,250100 |
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| 基金项目： | 山东省农业良种产业化工程项目 |
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| 摘 要： | 提取牛血液和精液DNA,进行PCR扩增、RFLP分析,建立尿苷酸合酶缺乏症(DUMPS)检测方法。对济南市6个奶牛场224头荷斯坦奶牛及53头种公牛进行了DUMPS的PCR-RFLP检测,共检测出2头杂合母牛(携带者),杂合子占检测母牛群的0.89%,在荷斯坦公牛中只检测到一种基因型,没有发现隐性突变基因的携带者。实验证明,该方法重复性好、灵敏性高、稳定性可靠。
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| 关 键 词： | 荷斯坦奶牛 |
| 文章编号： | 1004-4264（2006）03-006-02 |
| 收稿时间： | 2005-08-18 |
| 修稿时间： | 2005年8月18日 |
The Development of PCR-RFLP for Detecting Deficiency of Uridine Monophophate Synthase |
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| Wang Hongmei,Li Jianbin,Gao Yundong,Han Bo,Wang Changfa,Liu Wenhao,Zhong Jifeng.The Development of PCR-RFLP for Detecting Deficiency of Uridine Monophophate Synthase[J].China Dairy Cattle,2006(3):6-8. |
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| Authors: | Wang Hongmei Li Jianbin Gao Yundong Han Bo Wang Changfa Liu Wenhao Zhong Jifeng |
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| Institution: | 1.Dairy Cattle Center of Shandong Academy of Agricultural Sciences,Jinan 250100,China; 2.Shandong Ox Biotechnology Co.,Ltd.,Jinan 250100,China |
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| Abstract: | The Mothod for detecting DUMPS were developed by extracting DNA from blood and semen,performing PCR and RFLP.Blood samples from 224 Chinese Holstein dairy cattles in 6 herds from Jinan,semen samples from 53 Chinese Hostein bulls were tested to determine whether they are DUMPS-free or DUMPS-carriers,by PCR-RFLP.Two heterozygotes(DUMPS-carrier)were detected from Chinese Holstien dairy cattles.The DUMPS-carriers prevalence in Holstein dairy cattle were estimated to be 0.89%.only wild genotype was found in Chinese Holstein bulls,without the recessive DUMPS gene.The method have a strong repeatability,a high specificity,and good sensitivity. |
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| Keywords: | DUMPS PCR-RFLP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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