Assessment of a pyrogallol red technique for total protein measurement in the cerebrospinal fluid of dogs |
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Authors: | Behr S Trumel C Palanché F Braun J P |
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Institution: | Département des Sciences Biologiques et Fonctionnelles & UMR Physiopathologie et Toxicologie Expérimentales, Ecole Nationale Vétérinaire, 23 chemin des Capelles, 31076 Toulouse Cedex, France. |
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Abstract: | The measurement of protein concentration in the cerebrospinal fluid is a basic analytical method in neurology. In this study, a pyrogallol red technique using a human albumin calibrator previously validated in human medicine was tested for canine samples, and the results were compared with those obtained using urine test strips. Pyrogallol red significantly (P<0.05) but moderately underestimated purified dog albumin and globulins. The imprecision of the technique was low: intra- and between-series coefficients of variation were 1.6 and 4.3 per cent at protein concentrations of about 0.3 g/litre. Over 49 samples, there was good agreement between the pyrogallol red and test strip results (r=0.63), especially for low and high protein concentrations, but misclassifications were observed with '+' test strip readings. |
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