| 猪瘟特异性转移因子的制备及免疫活性鉴定 |
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| 引用本文： | 元永平,张佩君,陈德坤.猪瘟特异性转移因子的制备及免疫活性鉴定[J].动物医学进展,2007,28(2):5-7. |
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| 作者姓名： | 元永平 张佩君 陈德坤 |
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| 作者单位： | 1. 西北农林科技大学动物科技学院,陕西,杨陵,712100
2. 陕西省教育学院生物系,陕西,西安,710061 |
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| 基金项目： | 西北农林科技大学博士专项科研基金 |
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| 摘 要： | 为制备猪瘟特异性转移因子(CSFV-STF)并鉴定其免疫学活性,用猪瘟兔化弱毒和石门系强毒(CSFV-Shimen)配合免疫健康猪,取猪的脾脏,制备猪瘟特异性转移因子,并采用改良白细胞黏附抑制试验检测其免疫活性.结果显示,猪瘟特异性转移因子的LAI%为76.0%,显著高于对照组(P＜0.01).表明猪瘟特异性转移因子具有特异性免疫活性.
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| 关 键 词： | 猪瘟 特异性转移因子 白细胞黏附抑制试验 |
| 文章编号： | 1007-5038（2007）02-0005-03 |
| 收稿时间： | 2006-11-09 |
| 修稿时间： | 2006年11月9日 |
Preparation and Immune Activity Identification of CSFV-STF |
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| YUAN Yong-ping,ZHANG Pei-jun,CHEN De-kun.Preparation and Immune Activity Identification of CSFV-STF[J].Progress In Veterinary Medicine,2007,28(2):5-7. |
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| Authors: | YUAN Yong-ping ZHANG Pei-jun CHEN De-kun |
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| Abstract: | To prepare CSFV-STF and identify its immune activity by centrifugation and ultrafiltration,CSFV-STF was extracted from the porcine spleens,which had been immunized with recombined CSFV-Shimen strain.The immune activity was detected by leukocyte adherence inhibition test.Physicochemical properties and bioactivities of CSFV-STF were accorded with the standard of biological product.The LAI mouse of experimental group was significantly higher than that of control groups(P<0.01).CSFV-STF has a specific immune activity. |
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| Keywords: | Classical swine fever specific transfer factor leukocyte adherence inhibition test |
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