| 苹果优良矮砧S_(18)、s_(20)微茎尖脱毒组织培养技术研究 |
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| 引用本文： | 朱文勇,赵玉军,郭黄平,杜学梅,贺小红.苹果优良矮砧S_(18)、s_(20)微茎尖脱毒组织培养技术研究[J].果树学报,1995(4). |
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| 作者姓名： | 朱文勇 赵玉军 郭黄平 杜学梅 贺小红 |
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| 作者单位： | 山西省农科院果树研究所!太谷030800 |
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| 摘 要： | 通过对武乡海棠(Malus honanensis Rehd.)优良矮砧S_(18)、S_(20)两个单系的0.2mm微茎尖脱毒组织培养技术研究,解决了微茎尖切割、接种、分化及生根移栽等一系列技术难题,获得了S_(20)、S_(18)一批脱毒试管苗及移栽壮苗.分化数分别达2.0和3.6,生根率达76.1%和90.0%,平均根长2cm以上,平均根数5条,移栽成活率达78%.
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| 关 键 词： | 武乡海棠 微茎尖 脱毒 分化 生根 移栽 |
Techniques for Eliminating the Virus by Microstem Tissue Culture for Superior Apple Dwarf Rootstocks S_(18) and S_(20) |
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| Zhu Wenyong,Zhao Yujun,Guo Huangping,Du Xuemei,and He Xiaohong.Techniques for Eliminating the Virus by Microstem Tissue Culture for Superior Apple Dwarf Rootstocks S_(18) and S_(20)[J].Journal of Fruit Science,1995(4). |
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| Authors: | Zhu Wenyong Zhao Yujun Guo Huangping Du Xuemei and He Xiaohong |
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| Abstract: | Two tenth milimeter microstem of M. honanensis Rehd. superior apple dwarf rootstock S18 and S20 were collected during shoot growth period, sterilized, and cultured in the MS medium. A Series of trouble occurred during cultivation, including the excision and inoculation of the microstem, shoot differentiation, rooting, devitrification, and transplantating, were overcomed. The virus-free planlets were obtained which were identified by indicative plants. The rate of proliferation reached 2. 0-3. 6; percentage of rooting reached 76. 1%-90. 2%; root number was 5 per planlet, mean root length was 2 cm; survival percentage of transplantation reached 78%. |
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| Keywords: | M honanensis Rehd Microstem Eliminating the virus Differentiation Rooting Transplantation |
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