蝗虫节律基因pdp的克隆及功能分析

[目的]研究不同条件下蝗虫（Locusta migratoria）节律基因pdp（Pyruvate dehydrogenase phosphatase）的mRNA表达水平,进一步揭示蝗虫节律的分子机制。[方法]通过中国科学院动物研究所内的转录组数据库中找出pdp基因的原型来设计引物,以群居型东亚飞蝗的头部的反转录cDNA为模板,克隆出pdp基因的全序列。把一天24 h等分成8个点,进行定时取样,以qRT-PCR技术进行不同时段pdp基因表达量的测定;并在不同处理条件下。测定pdp基因的表达量。[结果]节律基因pdp在蝗虫的不同处理条件下变化不大。[结论]该研究对了解飞蝗节律规律、种群特点及有效防虫具有重要的现实意义。

Clone and Functional Analysis of Circadian Gene pdp in Locusta migratoria

[Objective]Through studying mRNA expression levels of circadian gene pdp in Locusta migratoria under different conditions to reveal molecular mechanisms of rhythm of Locusta migratoria.[Methods] Based on the database of Institute of Zoology,Chinese Academy of Sciences,to find out the prototype of the pdp gene and design primers.Using aggregated locust head cDNA cloning for templates,the complete sequence of the gene pdp was got.By dividing a day(24 hours)into eight points,we take fixed-point samples,and then measure the gene expression using qRT-PCR for different times of day.And then in different processing conditions,the pdp gene expression levels were determined.[Results] The rhythms of gene pdp locust changed slightly under different conditions.[Conclusion] The study had extremely realistic significance for understanding the rhythm and population characteristics of Locusta migratoria as well as effective insect-resistant.