| 剑麻叶片内源激素高效液相色谱测定方法研究 |
| |
| 引用本文： | 俞奔驰,黄富宇,吕平,梁立娟,彭静茹,张小玲. 剑麻叶片内源激素高效液相色谱测定方法研究[J]. 安徽农业科学, 2011, 39(22): 13266-13267,13289 |
| |
| 作者姓名： | 俞奔驰 黄富宇 吕平 梁立娟 彭静茹 张小玲 |
| |
| 作者单位： | 广西壮族自治区木薯研究所,广西南宁53000;广西农垦山圩农场,广西扶绥,532112;广西壮族自治区亚热带作物研究所,广西南宁,530001 |
| |
| 基金项目： | 广西科学资金项目(桂科青0728048) |
| |
| 摘 要： | [目的]探讨剑麻叶片内源激素的高效液相色谱（HPLC）测定方法。[方法]融合酶联免疫法（ELISA）的激素提取方法,对剑麻成熟叶片内源激素——赤霉素（GA3）、3-吲哚乙酸（IAA）、脱落酸（ABA）和玉米素（ZT）进行了HPLC测定。[结果]该流程可联合测定GA3I、AA、ABA和ZT,回收率分别为78.81%、104.96%8、1.24%和100.55%,且保留时间稳定,试验重现性好。[结论]整个提取、分离、分析流程较快速、准确,适合剑麻成熟叶片内源激素的分析测定。
|
| 关 键 词： | 高效液相色谱 剑麻 成熟叶片 内源激素 测定方法 |
High Performance Liquid Chromatography Determination of Endogenous Hormones in Mature Leaves of Agavybrid No.11648 |
| |
| Affiliation: | YU Ben-chi et al(Guangxi Institute of Subtropical Crops,Nanning,Guangxi 530001) |
| |
| Abstract: | [Objective] The aim was to study the high performance liquid chromatography(HPLC) determination of endogenous hormones in agavybrid No.11648 leaves.[Method] Combining the hormone extraction methods of enzyme linked immunoassay,Gibberellin acid(GA3),3-indoleacetic acid(IAA),abscisic acid(ABA) and zeatin(ZT) in mature leaves of agavybrid No.11648 were determined by HPLC.[Result] The procedure could analyze the GA3,IAA,ABA and ZT in combination,and their recoveries were 78.81%,104.96%,81.24% and 100.55% respectively with stable retention time and good repeatability.[Conclusion] The procedure is simple,rapid and accurate,so it is suitable for the efficient determination of endogenous hormone in mature leaves of agavybrid No.11648. |
| |
| Keywords: | High performance liquid chromatography Agavybrid No.11648 Mature leaves Endogenous hormones Determination method |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
