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秦巴山区桑叶中芦丁提取工艺优化
引用本文:张艳艳,蒲秋燕,黄平,王黎涛,刘存芳.秦巴山区桑叶中芦丁提取工艺优化[J].安徽农业科学,2011,39(22):13372-13373.
作者姓名:张艳艳  蒲秋燕  黄平  王黎涛  刘存芳
作者单位:陕西理工学院化学与环境科学学院,陕西汉中,723000
基金项目:陕西理工学院2010年大学生创新实验项目(UIRP1029)
摘    要:目的]研究提取桑叶中芦丁的工艺条件。方法]在单因素试验的基础上,以乙醇浓度、料液比、浸提时间、浸提温度为因素,以桑叶中芦丁提取率为指标,采用正交试验法对桑叶中芦丁的提取工艺进行优选。结果]桑叶芦丁最佳提取工艺为A3B1C1D3,即超声时间为50 min,提取溶剂为60%乙醇,提取温度为30℃,料液比为1∶20。在此工艺条件下,芦丁吸光度达0.171。结论]该研究得到桑叶芦丁提取的最佳工艺,为更好地开发利用桑叶提供了参考。

关 键 词:桑叶  芦丁  含量  提取

Optimization of Extraction Conditions of Rutin from Morus alba L.Leaf in Qinling & Bashan Areas
Institution:ZHANG Yan-yan et al(School of Chemical & Environmental Sciences,Shaanxi University of Technology,Hanzhong,Shaanxi 723000)
Abstract:Objective] To obtain the optimal extraction conditions for rutin from Morus alba L.leaf.Method] Using ethanol concentration,solid-to-liquid ratio,extraction time and extraction temperature as four factors and extraction rate of rutin as an indicator,the extraction conditions were optimized by orthogonal test based on single factor test.Result] The optimum extracting combination for rutin from mulberry leaf was A3B1C1D3.In other words,the mulberry leaf extracted with 60% ethanol as solid to liquid ratio of 1∶20 at 30 ℃ for 50 min was highest in yield with absorbance of 0.171.Conclusion] The optimal extraction process was obtained in this work,and this research provided reference for better development and application of Morus alba L. leaf.
Keywords:Morus alba L  leaf  Rutin  Optimization of extraction process
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