高效液相色谱法测定消浊通淋颗粒中槲皮素和山奈素的含量

[目的]建立消浊通淋颗粒中槲皮素和山奈素含量的测定方法。[方法]采用高效液相色谱法,色谱柱为岛津VP-ODS c（250.0mm×4.6 mm5,μm）,流动相为甲醇-0.4%磷酸水溶液（48∶52）,流速为1.1 ml/min,检测波长为360 nm。[结果]槲皮素在0.075 2～0.270 5μg范围内呈现良好线性关系,加样回收率为99.5%,RSD为0.30%（n=6）;山奈素在0.113 0～0.406 8μg范围内呈现良好线性关系,加样回收率为99.7%,RSD为0.34%（n=6）。[结论]高效液相色谱法测定消浊通淋颗粒中槲皮素和山奈素的含量操作简单、准确、重复性好、精密度高,可以作为消浊通淋颗粒的质量控制方法。

Determination of Quercetin and Kaempferol Contents in Xiaozhuotonglin Granules by HPLC

[Objective] The research aimed to establish a method for the determination of quercetin and kaempferol contents in Xiaozhuotonglin granules.[Method] The contents of quercetin and kaempferol in Xiaozhuotonglin granules was determined by using HPLC.The separation was performed on SHIMADZU VP-ODS c(250.0 mm×4.6 mm,5 μm).The mobile phase was methanol-0.4% phosphoric acid solution(48∶52) at the flow rate of 1.1 ml/min.The defection wavelength was 360 nm.[Result] The linear range of quercetin was in the range of 0.075 2-0.270 5 μg,the addition recovery rate was 99.5%,RSD=0.30%(n=6),the linear range of kaempferol was in the range of 0.113 0-0.406 8 μg,the addition recovery rate was 99.7%,RSD=0.34%(n=6).[Conclusion] The method for the determination of quercetin and kaempferol in Xiaozhuotonglin granules by HPLC is simple,rapid,good repeatability and accurate,and it is suitable for the determination of Xiaozhuotonglin granules.