水稻Ds插入具芒突变体相关基因的功能预测

[目的]研究水稻Ds插入具芒突变体形成的分子机理。[方法]采用TAIL-PCR技术,从水稻Ds插入具芒突变体中克隆出Ds侧翼基因序列,分析被插入基因的结构,并分析预测基因编码的蛋白功能。[结果]Ds插入在具芒突变体7号染色体Os07g0588700基因前1 339 bp处。Ds插入位置的下游基因编码产物含一个锌指区CX2CX3FX5LX2HX3H,且含高度保守的QALGGH保守区,为水稻的单锌指蛋白。[结论]Ds转座元件插入基因组中,影响了编码锌指蛋白基因的表达调控,使突变体显示出具芒的表型。

Prediction of the Function of an Awn-Related Gene in Ds-inserted Awned Rice Mutant

[Objective]The study aimed to preliminarily analyze the molecular mechanism of the Ds-inserted awned rice mutant.[Method]After cloned the Ds-flanking sequences from the mutant by using TAIL-PCR amplification technique,the structure of the inserted gene was analyzed,and the function of the product encoded by the gene was predicted.[Result]Ds element was inserted 1 339 bp upstream of the rice predicted gene Os07g0588700 of rice chromosome 7.It was revealed that the deduced product was a member of rice C2H2-type zinc finger protein family with QALGGH conservative region.It was a single zinc finger protein of the rice.[Conclusion]The insertion of the Ds involved the expression and regulation of the gene,which encoded the single zinc finger protein,thus the mutant had the awn.