| 黄芪中黄芪甲苷的提取工艺优化 |
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| 引用本文： | 段静雨,李岩,么焕开,孙远峰,李典,魏群利. 黄芪中黄芪甲苷的提取工艺优化[J]. 安徽农业科学, 2011, 39(22): 13396-13397 |
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| 作者姓名： | 段静雨 李岩 么焕开 孙远峰 李典 魏群利 |
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| 作者单位： | 徐州医学院药学院,江苏徐州,221004;徐州医学院药学院,江苏徐州,221004;徐州医学院药学院,江苏徐州,221004;徐州医学院药学院,江苏徐州,221004;徐州医学院药学院,江苏徐州,221004;徐州医学院药学院,江苏徐州,221004 |
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| 基金项目： | 徐州医学院院长专项人才基金(09KJZ23);江苏高校优势学科建设工程资助项目 |
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| 摘 要： | [目的]优化黄芪（RADIX ASTRAGALI）中黄芪甲苷的提取工艺。[方法]利用均匀设计法,采用小型工业设备TS-NS提取浓缩机组对黄芪进行提取浓缩,考察乙醇浓度、固液比、提取时间及提取次数对黄芪甲苷提取率的影响,并用HPLC-ELSD测定黄芪甲苷的含量,筛选最佳工艺。[结果]黄芪中黄芪甲苷的最佳提取工艺：乙醇浓度为80%,固液比为1∶8,提取次数为3次,提取时间为1 h。[结论]与现有文献报道的方法相比,优选的黄芪甲苷提取工艺简便、可靠,适合工业化生产。
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| 关 键 词： | 黄芪 黄芪甲苷 均匀设计法 提取工艺 HPLC-ELSD |
Optimization of the Extraction Process of Astragaloside IV from ASTRAGALI RADIX |
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| Affiliation: | DUAN Jing-yu et al(School of Pharmacy,Xuzhou Medical College,Xuzhou,Jiangsu 221004) |
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| Abstract: | [Objective] To optimize the extraction process of astragaloside IV from ASTRAGALI RADIX.[Method] Using TS-NS extraction and concentration industrial equipment to extract ASTRAGALI RADIX,effects of ethanol concentration,solid-liquid ratio,extraction time and extraction times on the yield of astragaloside IV were respectively investigated by uniform design experiment.Taking the content of astragaloside IV determined by HPLC-ELSD as index,the extraction process was optimized.[Results] The optimum extraction process of astragaloside IV from ASTRAGALI RADIX was:ethanol concentration of 80%,solid-liquid ratio of 1∶8,extraction times of 3 and extraction time of 1 h.[Conclusion] The optimum process for extracting astragaloside IV was simple,reliable and more suitable for industrial production compared with the reported methods. |
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| Keywords: | ASTRAGALI RADIX Astragaloside IV Uniform design Extraction process HPLC-ELSD |
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