| 紫外-氯化锂复合诱变选育聚半乳糖醛酸酶高产菌株 |
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| 引用本文： | 黄乐平,汤锋,刘启阳,陈卫平. 紫外-氯化锂复合诱变选育聚半乳糖醛酸酶高产菌株[J]. 安徽农业科学, 2011, 39(22): 13275-13276 |
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| 作者姓名： | 黄乐平 汤锋 刘启阳 陈卫平 |
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| 作者单位： | 1. 江西农业大学生物科学与工程学院,江西南昌,330045
2. 江西省食品发酵研究所,江西宜春,336000 |
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| 摘 要： | [目的]研究聚半乳糖醛酸酶高产菌株的复合诱变条件。[方法]黑曲霉孢子经紫外线辐射2 min后,涂布于含0.8%LiCl的培养基上培养,经初筛和复筛后测定聚半乳糖醛酸酶活力。[结果]获得1株产聚半乳糖醛酸酶活力为10.450万U/ml的菌株,产酶活力比出发菌株提高了60.77%。[结论]产聚半乳糖醛酸酶的黑曲霉经紫外线辐射2 min、0.8%LiCl复合诱变后,产酶活力提高了60.77%。
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| 关 键 词： | 聚半乳糖醛酸酶 紫外线 氯化锂 |
The Screening of High Producing Strains Yielding PG Based on Compound Mutation of UV and LiCl |
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| Affiliation: | HUANG Le-ping et al(College of Biological Sciences and Bioengineering,Jiangxi Agricultural University,Nanchang,Jiangxi 330045) |
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| Abstract: | [Objective]The aim was to study the compound mutation conditions of high producing strains yielding PG.[Method] After radiating for 2 min,Aspergillus niger spores are spread on culture medium with 0.8% LiCl,then after first screening and second screening,enzyme activity of PG are measured.[Result] A bacterial strain with 10.450×104 U/ml enzyme activity of PG are obtained,its enzyme activity of PG is improved by 60.77% than original strains.[Conclusion] After radiating for 2 min,Aspergillus niger spores are spread on culture medium with 0.8% LiCl,its enzyme activity of PG is improved by 60.77%. |
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| Keywords: | Polygalacturonase Ultraviolet rays LiCl |
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