| 乌江网箱养殖中患病花鲈的细菌分离与分子鉴定 |
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| 引用本文： | 王庆容,宋培勇,杨秀荣.乌江网箱养殖中患病花鲈的细菌分离与分子鉴定[J].水利渔业,2010(1). |
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| 作者姓名： | 王庆容 宋培勇 杨秀荣 |
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| 作者单位： | 贵州省遵义师范学院生物系; |
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| 基金项目： | 贵州省遵义市科技局资助项目[遵市科合社字(2007)21号] |
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| 摘 要： | 利用微生物学常用的细菌分离、纯化方法从患病花鲈(Lateolabrax japonicus)分离出9个菌株,编号分别为Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ、Ⅶ、Ⅷ、Ⅸ。对9个菌株的16SrRNA基因序列进行PCR扩增,均得到约600bp的片段。将扩增结果进行测序,结果输入到NCBI(http://www.ncbi.nlm.nih.gov/)中,用BLAST工具对序列进行同源性比对分析。结果表明,Ⅰ为杀鲑气单胞菌,同源性98%;Ⅱ为不动菌属,同源性97%;Ⅲ为肺炎克雷伯氏杆菌,同源性98%;Ⅳ为嗜水气单胞菌,同源性99%;Ⅴ为希瓦氏菌,同源性99%;Ⅵ为腐生葡萄球菌,同源性99%;Ⅶ为枯草芽孢杆菌,同源性99%;Ⅷ为沙雷氏菌属,同源性95%;Ⅸ为杀鲑气单胞菌,同源性97%。
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| 关 键 词： | 花鲈 细菌 分离 分子鉴定 16S rRNA |
Isolation and Molecular Identification of Endophytic Bacteria from Cultured Lateolabrax japonicus in Wujiang River |
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| WANG Qing-rong,SONG Pei-yong,YANG Xiu-rong.Isolation and Molecular Identification of Endophytic Bacteria from Cultured Lateolabrax japonicus in Wujiang River[J].Reservoir Fisheries,2010(1). |
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| Authors: | WANG Qing-rong SONG Pei-yong YANG Xiu-rong |
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| Institution: | WANG Qing-rong,SONG Pei-yong,YANG Xiu-rong(Department of Biology,Zunyi Normal College,Zunyi Guizhou 563003,China) |
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| Abstract: | With the traditional microbiology method,the 9 strains of endophytic bacteria were isolated from Lateolabrax japonicus of net cage culture in Wujiang River. About 600bp fragments of the 9 strains were amplified by PCR using general primers of 16S rRNA. The sequences of 16S rRNA were determined by entering into the NCBI (http:www.ncbi.nlm.nih.gov/) and the sequence homology were analyzed by BLAST tool. The results showed that nine stains were Aeromonas salmonicida (homology of 98%),Acinetobacter (homology of... |
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| Keywords: | Lateolabrax japonicus Bacteria Isolation Molecular identification 16S rRNA |
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