Sequence-specific isotope effects on the cleavage of DNA by bleomycin |
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| Authors: | J W Kozarich L Worth B L Frank D F Christner D E Vanderwall J Stubbe |
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| Affiliation: | Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742. |
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| Abstract: | Bleomycin is a metal- and oxygen-dependent DNA cleaver. The chemistry of DNA damage has been proposed to involve rate-limiting abstraction of the 4'-hydrogen. A DNA fragment has been prepared that contains [4'-2H]thymidine residues of high isotopic content. Primary kinetic isotope effects have been directly observed at individual thymidine residues with DNA sequencing technology. |
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