| 绿色木霉合成纤维素酶部分培养条件的优化 |
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| 引用本文： | 邵喜霞,韩大勇,张力. 绿色木霉合成纤维素酶部分培养条件的优化[J]. 安徽农业科学, 2009, 37(23) |
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| 作者姓名： | 邵喜霞 韩大勇 张力 |
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| 作者单位： | 甘肃农业大学动物科学技术学院,甘肃兰州,730070;江苏畜牧兽医职业技术学院,江苏泰州,225300 |
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| 摘 要： | [目的]确定绿色木霉ZJ株产纤维素酶的最佳诱导时间和诱导物,为其实际应用提供条件。[方法]接种绿色木霉ZJ株7 d内,每天取培养物样品,采用3,5-二硝基水杨酸法检测产酶量。将绿色木霉ZJ株接种添加了不同碳源或氮源的基础培养基中,观察绿色木霉的生长情况,测定菌丝重量,检测不同培养时间的培养物中CMCase酶的产量。[结果]绿色木霉ZJ株的最适培养时间为72～96 h;绿色木霉在以单糖、双糖为碳源的培养基中均能迅速生长,CMCase酶产量在3～4 d时达到高峰,以纤维素粉的诱导效果最佳;以硫酸铵与酵母膏组成的复合氮源最适合绿色木酶ZJ菌丝的生长,产酶活力最高。[结论]接种后3～4 d收获绿色木霉ZJ株培养物可获得最大产酶量;以纤维素粉作为碳源,以硫酸铵与酵母膏组成的复合氮源为氮源,绿色木霉的产酶活力最高。
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| 关 键 词： | 绿色木霉 纤维素酶 诱导合成 碳源 氮源 |
Optimization of Some Culture Conditions for Biosynthesis of Cellulase by Trichoderma vivide |
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| SHAO Xi-xia et al. Optimization of Some Culture Conditions for Biosynthesis of Cellulase by Trichoderma vivide[J]. Journal of Anhui Agricultural Sciences, 2009, 37(23) |
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| Authors: | SHAO Xi-xia et al |
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| Affiliation: | SHAO Xi-xia et al(College of Animal Science , Technology,Gansu Agricultural University,Lanzhou,Gansu 730070) |
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| Abstract: | [Objective] To determine the best culture time and inducer for the biosynthesis of cellulase by Trichoderma vivide and thus provide the conditions for its practical application.[Method] Within the 7 d after the inoculation of Trichoderma vivide ZJ strain,the cultures were collected once every day,and the enzyme yield was respectively determined by 3,5-dinitrosalicylic acid assay.The Trichoderma vivide ZJ strain was inoculated into basal medium added by different types of carbon sources or nitrogen sources,a... |
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| Keywords: | Trichoderma viride Cellulase Induced synthesis Carbon source Nitrogen source |
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