对虾育苗水体副溶血性弧菌耐药性分析

为了解广东、广西沿海2010-2012年对虾育苗水体中副溶血性弧菌的耐药性变化,为育苗期对虾病害预防提供依据.研究依据GB/T4789.7-2008对各地育苗水体样品进行菌株分离、鉴定,并用纸片法进行耐药性检测.结果显示,72株在TCBS培养基上显示绿色的弧菌中检出副溶血性弧菌30株,检出率为41.7％,分离菌株对青霉素、氨苄西林、苯唑青霉素、米诺环素、阿莫西林、四环素、阿洛西林显示高度耐药,对四环素的耐药率达97％.对虾育苗水体在TCBS培养基上显示绿色的弧菌主要以副溶血性弧菌为主,所有菌株耐药性比较强,应加强育苗期抗生素使用的管理.

Antimicrobial resistance of Vibrio parahaemolyticus isolated from the pond water of shrimp larvae

To investigate the changes of the antimicrobial resistance of Vibrio parahaemolyticus isolated from the pond water of shrimp larvae in Guangdong and Guangxi coastal from 2010 to 2012, and offer the date for the disease prevention of shrimp larvae during the breeding period, the Vibrio parahaemolyticus strains from different regions were isolated and identified according to GB/T4789.7 - 2008, and the microbial sensitivity tests were performed with the Kirby - Bauer method. The results showed that, among the 72 Vibrio strains from the pond water which displayed green on the TCBS medium, 30 strains （41.7%） were Vibrio parahae- molyticus. The susceptibilities of the 30 strains to penicillin, ampicillin, benzene, minocyeline, amoxicillin azole penicillin, tetracycline, azlocillin showed highly resistant, prevalence of tetracycline resistance as high as 97%. The Vibrio strains from the pond water of shrimp larvae which displayed green on the TCBS medium mainly were Vibrio parahaemolyticus, all strains resistances were strong, and we should enhance the regula- tions of using antibiotics during the breeding period.