| 黑龙江省链格孢属病原菌的RAPD分析 |
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| 引用本文： | 姚亮亮,丁俊杰,张铉哲,顾鑫,杨晓贺,赵海红,申宏波,刘伟. 黑龙江省链格孢属病原菌的RAPD分析[J]. 中国马铃薯, 2013, 0(6): 365-369 |
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| 作者姓名： | 姚亮亮 丁俊杰 张铉哲 顾鑫 杨晓贺 赵海红 申宏波 刘伟 |
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| 作者单位： | [1]农业部佳木斯作物有害生物科学观测实验站,黑龙江省农业科学院佳木斯分院,黑龙江佳木斯154007 [2]东北农业大学农学院,黑龙江哈尔滨150030 [3]黑龙江农业职业技术学院,黑龙江佳木斯154007 |
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| 基金项目： | 国家农业部948项目“马铃薯地上垄体栽培模式配套与示范”（2011-Z52）. |
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| 摘 要： | 利用RAPD技术分别对分离自黑龙江省各地区的90株链格孢属病原菌进行了遗传多样性分析。利用NTSYS 2.0进行链格孢属病原菌的聚类分析。RAPD分析结果表明,引物OPE-19扩增后产生的各个群体间相似性系数分布在0.601.00。在68.0%相似性水平上可划分为3个主要RAPD组群和8个次要RAPD组群。聚类分析结果表明,黑龙江省链格孢属病原菌RAPD组群与寄主植物和分离地区之间没有相关性。
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| 关 键 词： | 链格孢属病原菌 RAPD 马铃薯早疫病菌 |
RAPD Analysis of Alternaria Pathogens in Heilongjiang Province |
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| Affiliation: | YAO Liangliang, DING Junjie ,ZHANG Xuanzhe , GU Xin ,YANG Xiaohe, ZHAO Haihong, SHEN Hongbo, LIU We ( 1. Observation and Experiment Station of Crop Pests of Jiamusi, Ministry of Agriculture/Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences, Jiamusi, Heilongjiang 154007, China; 2. College of Agronomy, Northeast Agricultural University, Harbin Heilongjiang 150030, China; 3. Heilongjiang Agricultural College of Vocational Technology, Jiamusi, Heilongjiang 154007, China ) |
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| Abstract: | To understand the genetic diversity of 90 Alternaria pathogen isolates col ected from Heilongjiang Province, the genotypic characteristics was performed by using random amplified polymorphic DNA （RAPD）, and cluster analysis of Alternaria pathogens was carried out by using NTSYs program. RAPD results indicated that the similarity coefficients of RAPD group amplified by the primer OPE-19 ranged from 0.60 to 1.00. According to RAPD analysis by using the primer OPE-19, 90 isolates were separated into three major RAPD groups and eight minor groups at the similarity level of 68.0%. The cluster analysis results suggested that RAPD groups not be correlated with the host plants and locations where the isolates were col ected. |
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| Keywords: | Alternaria pathogens RAPD Alternaria solani |
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