| HPLC快速测定绞股蓝中槲皮素的含量 |
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| 引用本文： | 王绍云,张文华,蒋天智,肖志友.HPLC快速测定绞股蓝中槲皮素的含量[J].安徽农业科学,2010,38(20):10641-10643. |
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| 作者姓名： | 王绍云 张文华 蒋天智 肖志友 |
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| 作者单位： | 凯里学院环境与生命科学学院,贵州凯里,556011;凯里学院环境与生命科学学院,贵州凯里,556011;凯里学院环境与生命科学学院,贵州凯里,556011;凯里学院环境与生命科学学院,贵州凯里,556011 |
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| 基金项目： | 凯里学院院级课题项目，凯里学院《植物学》重点学科建设资助项目 |
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| 摘 要： | 目的]建立绞股蓝Gynostemma pentaphyllum（Thunb.）Makino]中槲皮素含量的简便、快速、准确检测方法。方法]用Microsorb-MV100-5C18色谱柱（4.6mm×250.0mm,5μm）,ProStar325紫外可见检测器,流动相为甲醇-混合液（乙氰∶1.0%醋酸∶0.4%磷酸=30∶35∶35,V/V/V）（37∶63,V/V）,流速为0.6ml/min,检测波长为372nm,柱温为室温,进样量为20μl。结果]槲皮素在0.64~25.60μg/ml范围内与峰面积线性关系良好（r=0.9999）,方法平均回收率大于96%（n=9）。结论]该研究建立的方法简便、快速、准确。
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| 关 键 词： | 绞股蓝 槲皮素 高效液相色谱法 |
Rapid Determination of Quercetin Content in Gynostemma pentaphyllum (Thunb.) Makino by HPLC |
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| Institution: | WANG Shao-yun et al(College of Environment and Life Science of Kaili University,Kaili,Guizhou 556000) |
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| Abstract: | Objective] The study aimed to establish a simple,rapid and accurate analytical method for detecting quercetin content in Gynostemma pentaphyllum(Thunb.)Makino by high perfomance liquid chromatograph(HPLC).Method]The conditions of chromatography were as follows:Microsorb-MV100-5C18 column(4.6 mm × 250.0 mm,5 μm)and ProStar 325 UV-vis detector were adopted with a mobile phase of methanol-mixtrue(acetonitrile∶1% acetic acid∶0.4% phosphoric acid,V/V/V)(37∶63,V/V);the flow rate was set at 0.6 ml/min,and the detective wavelength was set at 372 nm.The column temperature was room temperature and the injection volume was 20 μl.Result]The linear range was 0.64-25.60 μg/ml(r=0.999 9),the average recovery was over 96%(n=9).Conclusion] The established method is simple,rapid and accurate. |
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| Keywords: | Gynostemma pentaphyllum(Thunb )Makino Quercetin HPLC |
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