灿烂弧菌的疏水性和生物被膜形成能力

以仿刺参化皮病病原菌—灿烂弧菌(Vibrio splendidus)AP622为试验菌株,研究了培养材料、培养时间、培养基、葡萄糖浓度对菌株生物被膜形成能力的影响,证实鞭毛和菌毛介导的运动性,同时比较了浮游细菌与形成生物被膜的细菌对抗生素的敏感性,并研究了菌株的疏水性。结果表明,菌株AP622为高疏水性和高生物被膜形成菌株,在聚氯乙烯为培养材料、含葡萄糖浓度为0.5%、LB培养基中形成的生物被膜最多,生物被膜形成周期为24 h。菌株AP622明显表现出鞭毛介导的群集性和Ⅳ型菌毛介导的颤搐等运动力。生物被膜中的菌株AP622对抗生素的抵抗力明显强于浮游细菌,其最小抑菌浓度(MIC)是浮游细菌的32倍。综上所述,菌株AP622具有很强的疏水性和高生物被膜形成能力,判断其具有很强的黏附力,同时具有明显的抗药性。

Cell surface hydrophobicity and biofilm formation ability of Vibrio splendidus

Vibrio splendidus infects a range of hosts,including fish,shellfish,and echinoderms.Infection with this pathogen has led to significant economic loss in several cultured species,including the sea cucumber(Aposti-chopus japonicus).Thus,there is an urgent need to understand the pathogenesis of V.splendidus.The virulence of a pathogen is partly a function of its adhesion properties.Adhesion depends on cell surface hydrophobicity and biofilm formation.The hydrophobic cell surface provides an advantage to the ba...