花叶锦带花组织培养的研究

以花叶锦带花的幼嫩茎段为外植体,进行组织培养与快速繁殖试验。研究结果表明:(1)初代培养的适宜培养基为MS+6-BA 0.50 mg/L+IBA 0.05 mg/L,附加480 mg/L氨苄青霉素钠,可有效抑制内生菌的污染,抑菌率达93.3%;(2)适宜增殖的培养基为MS+6-BA 0.50 mg/L+NAA 0.05 mg/L,增殖系数达6.70,苗健壮,无玻璃化现象;(3)生根最佳培养基为1/2MS+IBA 0.50 mg/L,生根率达98%,根系粗壮。

Stem-segment Culture and Propagation of Weigela florida cv. Variegata

The young stem-segment with axillary buds was used as the explant,the propagation by tissue culture of Weigela florida cv.Variegata was studied.The results showed that the media coutaining 480mg/L sodium ampicillin was helpful to reduce the propagation of plant endophyte.The appropriate media for germination of the axillary buds was MS added 6-BA(0.50mg/L) and IBA(0.05mg/L).The subculture media for plant differentiation was MS added 6-BA(0.50mg/L) and NAA(0.05mg/L),the high coefficient of propagation was 6.70.At the same time,the plants cultured in tube had not the vitric-plant phenomena.For plant rooting,the appropriate media was 1/2MS added IBA(0.50mg/L),which could brought on the high rooting rate at 98%.