The influence of supplemental alpha-galactosidase and phytase in a vegetable ration for dogs on the digestibility of organic components and phytate phosphorus

The use of dry feeds for dogs is increasing for different reasons: in comparison to wet canned feed it is easier to handle, the amount to be given is smaller and the daily cost is less. In producing a dry pellet more vegetable ingredients are used that can contain several antinutritional factors. Some of them can be eliminated by heating. Others like galactosides and phytates are heat-resistant but can be broken down by adding the appropriate enzymes. The specific enzymes are indeed not secreted by the animal itself. In farm animals, e.g. pigs, alpha-galactosidase has been introduced in order to enhance the energetic value of these alphagalactosides (Veldman et al. 1993). In companion animals this enzyme is more often indicated as an antiflatulence agent, because the main alpha-galactosides present in legume seeds (stachyose, raffinose) are known as flatulence components (Fleming 1981). These oligosaccharides do not necessarily imply an overall lower nutrient digestibility. Zuo et al. (1996) did not prove a significant difference in digestibility between conventional and low oligosaccharide soybean meal in dog rations. Others however, (Wiernusz et al. 1995) reported the highest digestibilities and the best stool quality of those rations that contained the least amount of soybean oligosaccharides. On the other hand, phytates (phospho-inositols) are known as complexing agents of several divalent cations (Fe, Mg, Zn, Ca) and are themselves considered as a phosphorus source, unavailable to monogastric animals. Phytase is the appropriate enzyme, which is not secreted by the animal itself, but, when added to the diet, can enhance the bio-availability of several minerals and trace elements (Rimbach et al. 1994) by hydrolysing the phospho-inositol molecule. The aim of the experiment, reported here, is to investigate the influence of the addition of alpha-galactosidase, phytase, or both to a nearly complete vegetable ration, consisting of a relatively high content of galactosides and phytates. The combination of both enzymes was carried out because Wenk et al. (1993) reported a possible interaction between a carbohydrase and a phytase enzyme in pigs.