山羊体细胞重编程过程中慢病毒对细胞的毒性作用

目的]检测慢病毒对普通山羊、克隆奶山羊和转基因克隆奶山羊的作用.方法]采用慢病毒作为载体感染普通山羊、克隆奶山羊(♂12001)和转基因克隆奶山羊(♀12003)体细胞,通过细胞生长曲线,检测慢病毒对3种山羊细胞的毒性作用.结果]感染后的3种体细胞在感染前3 d,细胞大量凋亡,4~7 d筛选出的细胞大量增殖.未经慢病毒感染的3种细胞接种2 d后数量开始明显增加,第25天进入对数生长期,第57天进入平台期,呈现典型的"潜伏期-对数生长期-停滞期"生长模式.结论]慢病毒对细胞有毒性作用,会引起细胞大量凋亡.

Cytotoxicity of Lentivirus to Goat during Somatic Reprogramming

Objective] The aim is to detect the role of lentivirus in normal goats, cloned dairy goats and transgenic cloned dairy goats. Meth-ods] Using lentiviral vector infection as a common goat, cloned goats (male 12001) and transgenic cloned goats (female 12003) somatic cells by cell growth curve, toxic effect of lentivirus on three kinds of goat cell was detected . Result] After infection, the three somatic cells were infected with 3 days, and the apoptosis of cells was large. The cells screened from 4 d to 7d proliferated greatly. Without the lentivirus infected three cells significantly increased after inoculation with 2 d, cells entered the logarithmic growth phase from 2rd day to 5th day, then entered the platform phase from 5th day to 7th day, it was a typical "latency-logarithmic growth period - the period of stagnation" growth mode. Conclusion] Lentivirus plays a toxic role in cells and can cause apoptosis in a large number of cells.