氮素对大麦籽粒中酚酸和蛋白质含量的影响

采用穗培养技术在7个氮素(NH4NO3为氮源)浓度水平(N1:0mg/L;N2:412.5 mg/L;N3:825.0mg/L;N4:1237.5mg/L;N5:1650.0ms/L;N6:3300.0mg/L;N7:4950.0mg/L)下研究了氮素对2个大麦品种籽粒中酚酸和蛋白质含量的影响.结果表明,在高氮水平(N...

EFFECTS OF NITROGEN ON PHENOLIC ACID AND PROTEIN CONTENT IN BARLEY(Hordeum vulgare L.)GRAIN

Nitrogen is one of the necessary nutrient elements for crop growth. The primary and secondary metabolisms of plant are regulated by nitrogen nutrient levels. As important secondary metabolites, phenolic acids play significant roles in plant resistances, and potentially contribute to barley qualities. However, little is known concerning effects of nitrogen on barley (Hordeum vulgare L.) phenolic acids. In the present study, two barley cultivars were used, and seven nitrogen concentration treatments (0, 412.5, 825.0, 1237.5, 1650, 3300, and 4950mg/L, respectively) under in vitro spike culture were conducted to investigate the effects of nitrogen on phenolic acid and protein contents in barley grain. Results showed that the development of barley grain was inhibited under high nitrogen levels (3300~4950mg/L), and grain weight decreasing significantly was as the response. Total hydroxybenzoic acid content (THBA), total hydroxycinnamic acid content (THCA), total phenolic acid content (TPA) and THBA/THCA ratio were all significantly affected by nitrogen concentration, cultivar and the interaction between nitrogen concentration and cultivar. Four indexes above were all significantly positively correlated with nitrogen concentration. Except for hordein content (HC), the protein indexes of crude protein content (CPC), salt-soluble protein content (SSPC), and glutelin content (GC) were all significantly affected by nitrogen concentration, moreover, SSPC and GC were significantly affected by cultivar and the interaction between nitrogen concentration and cultivar. Excluding HC and GC in the four indexes, CPC and SSPC were significantly positive\|correlated with nitrogen concentration. The results also indicated that phenolic acid contents (including THBA, THCA, and TPA) in barley grain increased cooperated with CPC and SSPC when the nitrogen nutrient level went up.