KM和BALB/c小鼠痘病毒感染的诊断

20只外购的KM和BALB/c小鼠发生了酷似鼠脱脚病的典型症状，取病鼠的肝、脾、肾、淋巴结和脑作组织学检查，发现其病理变化呈痘病毒感染的特征性改变，组织镁浆液接种传代的BHK细胞，可发生明显的CPE变化，经电镜超薄切征及负染检查发现大量的痘病毒颗粒，粒子的中心为DNA核酸和蛋白质组成的拟核，在衣壳的外面有囊膜包裹，对鸡红细胞均呈阳性凝集反应（HA为1：16），用小鼠痘病毒ELISA试剂盒检测病鼠血清呈阳性反应，用病毒悬液免疫接种KM和BALB／C小鼠后，发现前者感染性强，发病快，症状典型，以急性为主，并迅速出现死亡。后者对鼠痘抵抗力强，一般呈陷性感染，特征性症状出现较缓慢，但后者一旦施加实验因素动物则出现症状，也可迅速死亡。实验证明，该群小鼠患的传染病病原为鼠痘病毒。

Observation on the Poxvirus Infection in KM and BALB/c Mice

An outbreak of poxvirus infection was occurred in twenty bought KM and BALB/c mice .The livers,spleens, kidneys, lymph nodes and cerebrum of ill mice were used to histology observation. The characteristic of pathologic change was poxvirus infection .when the tissue homogenate fluid infected passaging BHK cell, we could see apparent CPE change. A lot of poxvirus particle were found in EMP. The center of particle was nucleoid composed by DNA and protein ,The agglutination reaction was positive (HA=1:16).By ELISA kit examination the serum of ill mice expressed poxvirus antibody positive reaction, After suspension of virus infected KM and BALB/c mice .It was found that the former was easily infected, with apparent symptom .The latter had strong resistance, latent infection. The symptom always occurred slowly, but when experiment factors were exerted,the BALB/c mice quickly had symptom, and died. It proved that the pathogen was poxvirus .