| 利用DNA shuffling技术构建含5个cry基因的突变体库以筛选高毒力杀虫蛋白 |
| |
| 引用本文： | 张小琼,林拥军.利用DNA shuffling技术构建含5个cry基因的突变体库以筛选高毒力杀虫蛋白[J].华中农业大学学报,2011,30(1):13-17. |
| |
| 作者姓名： | 张小琼 林拥军 |
| |
| 作者单位： | 华中农业大学生命科学技术学院,武汉,430070 |
| |
| 摘 要： | 以改造合成的5个Bt基因(crylC*、cry2A*、crylAb、crylAc和fry9C*)为材料,利用DNA shuff-ling的体外分子进化技术构建1个含有1 000个重组基因克隆的大肠杆菌表达库;通过诱导表达重组蛋白.ELISA方法测定蛋白表达量,以棉铃虫(Helicoverpa armigera)为试验昆...
|
| 关 键 词： | DNA shuffling Cry蛋白 序列比较 突变体库 |
Constructing cry genes mutant library with DNA shuffling method for screening high toxic Cry protein |
| |
| ZHANG Xiao-qiong,LIN Yong-jun.Constructing cry genes mutant library with DNA shuffling method for screening high toxic Cry protein[J].Journal of Huazhong Agricultural University,2011,30(1):13-17. |
| |
| Authors: | ZHANG Xiao-qiong LIN Yong-jun |
| |
| Institution: | ZHANG Xiao-qiong LIN Yong-jun College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China |
| |
| Abstract: | An E.coli-expression mutant library containing 1 000 cry mutant genes was constructed using DNA family shuffling method with five initial cry genes cry1Ac,cry1Ab,cry1C*,cry2A* and cry9C*.The 1 000 clones were expressed in E.coli and their toxicity was tested upon Helicoverpa armigera(cotton bollworm) larvae.The expression level of mutation clones were quantified by ELISA.Compared with the original Cry1Ac,there were 122 clones with significantly enhanced toxicity,38 clones with similar toxicity;232 clones wi... |
| |
| Keywords: | DNA shuffling |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《华中农业大学学报》浏览原始摘要信息 |
| 点击此处可从《华中农业大学学报》下载免费的PDF全文 |
